Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain

A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased i...

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Bibliographic Details
Published in:Bioresource technology 2011-02, Vol.102 (4), p.3918-3922
Main Authors: Oh, Baek-Rock, Seo, Jeong-Woo, Heo, Sun-Yeon, Hong, Won-Kyung, Luo, Lian Hua, Joe, Min-ho, Park, Don-Hee, Kim, Chul Ho
Format: Article
Language:English
Subjects:
Aldehyde Dehydrogenase - chemistry
Biological and medical sciences
Biotechnology
Biotechnology - methods
Butylene Glycols - chemistry
Ethanol
Ethanol - chemistry
Ethanol production
Ethyl alcohol
Fermentation
Fundamental and applied biological sciences. Psychology
Gamma Rays
Glycerol
Glycerol - chemistry
Glycerols
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Lactates
Lactates - chemistry
Lactic Acid - analogs & derivatives
Lactic Acid - chemistry
Metabolites
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Mutant
Mutation
Pathways
Propylene Glycols - chemistry
Pyruvate Decarboxylase - chemistry
Pyruvates
Strain
Succinic Acid - chemistry
Zymomonas - enzymology
Zymomonas mobilis
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Summary:A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased in the GEM167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-BD), ethanol, lactate, and succinate, were increased. Notably, ethanol production from glycerol was greatly enhanced upon fermentation by the mutant strain, to a maximum production level of 21.5g/l, with a productivity of 0.93g/l/h. Ethanol production level was further improved to 25.0g/l upon overexpression of Zymomonas mobilispdc and adhII genes encoding pyruvate decarboxylase (Pdc) and aldehyde dehydrogenase (Adh), respectively in the mutant strain GEM167.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.12.007