Production of indole-3-acetic acid and related indole derivatives from L-tryptophan by Rubrivivax benzoatilyticus JA2

Rubrivivax benzoatilyticus JA2 produces indoles with simultaneous utilization of L-tryptophan. Fifteen chromatographically distinct indole derivatives were detected from the L-tryptophan-supplemented cultures of R. benzoatilyticus JA2. Nine of these were identified as, indole 3-acetamide, Methoxyind...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2011-02, Vol.89 (4), p.1001-1008
Main Authors: Mujahid, Md, Sasikala, Ch, Ramana, Ch. V
Format: Article
Language:English
Subjects:
Acetic acid
Bacteria
Betaproteobacteria - metabolism
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnological Products and Process Engineering
Biotechnology
Carboxylic acids
Culture Media - chemistry
Enzymes
Experiments
Fundamental and applied biological sciences. Psychology
Growth conditions
Hormones
Indole
Indole 3-acetic acid
Indoleacetic Acids - metabolism
Indoles
L-Tryptophan
Life Sciences
metabolism
Metabolite probe
Metabolites
Microbial Genetics and Genomics
Microbiology
Nitrogen
Plant growth
Rubrivivax benzoatilyticus
Sensors
Stable isotopes
Studies
Tryptophan - metabolism
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Summary:Rubrivivax benzoatilyticus JA2 produces indoles with simultaneous utilization of L-tryptophan. Fifteen chromatographically distinct indole derivatives were detected from the L-tryptophan-supplemented cultures of R. benzoatilyticus JA2. Nine of these were identified as, indole 3-acetamide, Methoxyindole-3-aldehyde, indole 3-aldehyde, methoxyindole-3-acetic acid, indole 3-acetic acid, indole-3-carboxylic acid, indole-3-acetonitrile, indole, and trisindoline. Tryptophan stable isotope feeding confirmed the indoles produced are from the supplemented L-tryptophan. Indole 3-acetic acid is one of the major products of L-tryptophan catabolism by R. benzoatilyticus JA2 and its production was influenced by growth conditions. Identification of indole 3-acetamide and tryptophan monooxygenase activity suggests indole 3-acetamide routed IAA biosynthesis in R. benzoatilyticus JA2. The study also indicated the possible multiple pathways of IAA biosynthesis in R. benzoatilyticus JA2.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-010-2951-2