Kinetics of Lime Pretreatment of Sugarcane Bagasse to Enhance Enzymatic Hydrolysis

• Published in: Applied biochemistry and biotechnology 2011-03, Vol.163 (5), p.612-625
• Main Authors: Fuentes, Laura L. G, Rabelo, Sarita C, Filho, Rubens Maciel, Costa, Aline C
• Format: Article
• Language: English
• Subjects: Bagasse; beta-Glucosidase - metabolism; Biochemistry; Biological and medical sciences; Biomass; Biotechnology; Biotechnology - methods; Calcium Compounds - chemistry; Cellulase - metabolism; Cellulose; Cellulose - chemistry; Cellulose - metabolism; Chemistry; Chemistry and Materials Science; Enzyme kinetics; Fundamental and applied biological sciences. Psychology; Hydrolysis; Kinetics; Lime treatment; Oxides - chemistry; Saccharum - chemistry; Sugarcane
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-010-9067-0

The objective of this work was to determine the optimum conditions of sugarcane bagasse pretreatment with lime to increase the enzymatic hydrolysis of the polysaccharide component and to study the delignification kinetics. The first stage was an evaluation of the influence of temperature, reaction time, and lime concentration in the pretreatment performance measured as glucose release after hydrolysis using a 2³ central composite design and response surface methodology. The maximum glucose yield was 228.45 mg/g raw biomass, corresponding to 409.9 mg/g raw biomass of total reducing sugars, with the pretreatment performed at 90°C, for 90 h, and with a lime loading of 0.4 g/g dry biomass. The enzymes loading was 5.0 FPU/dry pretreated biomass of cellulase and 1.0 CBU/dry pretreated biomass of β-glucosidase. Kinetic data of the pretreatment were evaluated at different temperatures (60°C, 70°C, 80°C, and 90°C), and a kinetic model for bagasse delignification with lime as a function of temperature was determined. Bagasse composition (cellulose, hemicellulose, and lignin) was measured, and the study has shown that 50% of the original material was solubilized, lignin and hemicellulose were selectively removed, but cellulose was not affected by lime pretreatment in mild temperatures (60-90°C). The delignification was highly dependent on temperature and duration of pretreatment.