Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) Producer Isolated from an Antarctic Environment

A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported...

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Bibliographic Details
Published in:Current microbiology 2009-11, Vol.59 (5), p.514-519
Main Authors: López, Nancy I, Pettinari, M. Julia, Stackebrandt, Erko, Tribelli, Paula M, Põtter, Markus, Steinbüchel, Alexander, Méndez, Beatriz S
Format: Article
Language:English
Subjects:
Acidification
Antarctic Regions
Bacteria
Bacteriology
Biochemical tests
Biomedical and Life Sciences
Biotechnology
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Hydroxybutyrates - metabolism
Life Sciences
Microbiology
Molecular Sequence Data
Nitrate reduction
Phylogeny
Poly-3-hydroxybutyrate
Polyesters - metabolism
Ponds
Pseudomonas
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Pseudomonas veronii
RNA, Ribosomal, 16S
Taxonomy
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Summary:A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, ß-galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-009-9469-9