Preferential ammonium uptake during growth cycle and identification of ammonium transporter genes in young pear trees

Five sequences showing homology with gene coding for ammonium (NH+ 4 ) transporters of the subfamily AMT1 (AMT1 genes) involved in the nitrogen (N) uptake process were identified in 2-year-old ‘Rocha’ pear/BA29 through reverse transcription polymerase chain reaction (RT-PCR) analysis. Multiple AMT1...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant nutrition 2011-01, Vol.34 (6), p.798-814
Main Authors: Mota, Mariana, Neto, Claudia B, Monteiro, Antonio A, Oliveira, Cristina M
Format: Article
Language:English
Subjects:
absorption
Agronomy. Soil science and plant productions
ammonium nitrate
ammonium transporters
Biological and medical sciences
Economic plant physiology
fertilizer-use-efficiency
Fundamental and applied biological sciences. Psychology
genes
gibberellic acid
leaves
Metabolism
Metabolism. Physicochemical requirements
nitrogen
nitrogen fertilizers
nutrient use efficiency
Nutrition. Photosynthesis. Respiration. Metabolism
pears
Plant physiology and development
pruning
Pyrus communis
reverse transcriptase polymerase chain reaction
shoots
transporters
Trees
vegetative growth
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Five sequences showing homology with gene coding for ammonium (NH+ 4 ) transporters of the subfamily AMT1 (AMT1 genes) involved in the nitrogen (N) uptake process were identified in 2-year-old ‘Rocha’ pear/BA29 through reverse transcription polymerase chain reaction (RT-PCR) analysis. Multiple AMT1 genes were expressed in each phase of the growth cycle and results suggest that ammonium uptake is conducted by several transporters expressed simultaneously. Trees grown in pots received 2 g N tree−1, at dates corresponding to active shoot vegetative growth, shoot growth cessation and beginning of leaf fall, in the form of either ammonium nitrate (NH 15 4 NO3) or 15NH4NO3. N was preferentially absorbed as NH+ 4 at all sampling dates, ranging from 62% to 66% of the total labelled N recovered in the trees. The fertilizer N use efficiency was also higher for ammonium treatment than for nitrate treatment at all sampling dates. N uptake rate and preferential NH+ 4-N absorption were not altered in response to root pruning and gibberellic acid (GA3) treatments.
ISSN:0190-4167
1532-4087
1532-4087
DOI:10.1080/01904167.2011.544350