Effects of transgenic Bt cotton on overwintering characteristics and survival of Helicoverpa armigera

• Published in: Journal of insect physiology 2011, Vol.57 (1), p.153-160
• Main Authors: Ouyang, Fang, Liu, Zhudong, Yin, Jin, Su, Jianwei, Wang, Chenzhu, Ge, Feng
• Format: Article
• Language: English
• Subjects: adults; Animals; Bacillus thuringiensis; Bacterial Proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bt cotton; Cold Temperature; Cold-hardiness; cotton; Diapause; diet; Endotoxins; Endotoxins - genetics; Endotoxins - metabolism; Feeding Behavior; Female; genetics; glycogen; Gossypium; Gossypium - genetics; Gossypium - metabolism; Gossypium - parasitology; growth & development; Helicoverpa armigera; Hemolysin Proteins; Hemolysin Proteins - genetics; Hemolysin Proteins - metabolism; laboratory experimentation; Larva; Larva - growth & development; Larva - physiology; larvae; Lepidoptera; lipids; Male; metabolism; methods; Moths; Moths - growth & development; Moths - physiology; Noctuidae; overwintering; parasitology; Pest Control, Biological; Pest Control, Biological - methods; physiology; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - parasitology; Pupa; Pupa - growth & development; Pupa - physiology; pupae; Pupal survival; pupation; Seasons; supercooling; transgenic plants; trehalose
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2010.10.007

[Display omitted] ▶ Bt cotton significantly decreased the incidence of pupation and diapause incidence of H. armigera in the over-wintering generation. ▶ Survival of diapausing pupae and emergence of adults from larvae fed on Bt cotton was significantly lower than those reared on non-Bt cotton. ▶ A Bt cotton diet weakened preparedness of the cotton bollworm for overwintering. ▶ Bt cotton depressed emergence of overwintering generation adults and in turn reduced density of the first generation the following year. The effects of transgenic Bt cotton on the overwintering generation of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), are unknown. We hypothesized that a Bt cotton diet may adversely affect fitness of this generation and examined fresh weight, lipids, glycogens, low-molecular-weight sugars and SCPs (supercooling points) of pupae, as well as survival of larvae, diapausing pupae and adult emergence in comparison with controls. Field and laboratory experiments showed that larvae fed on Bt cotton had a decreased pupation rate, and fewer entered diapause and emerged as adults compared with larvae fed non-Bt cotton. Furthermore, larvae fed Bt cotton had reduced pupal weight, glycogen content and trehalose levels both in diapausing and in non-diapausing pupae, and only diapausing pupae had an increased SCP compared to controls. The SCPs of diapausing pupae reared on Bt cotton were significantly higher than those reared on non-Bt cotton. The trehalose levels of diapausing pupae reared on Bt cotton were significantly lower than those of larvae reared on non-Bt cotton. Thus, these results suggest that a Bt cotton diet weakens the preparedness of cotton bollworm for overwintering and reduces survival of the overwintering generation, which will in turn reduce the density of the first generation in the following year. Effects of transgenic Bt cotton on the overwintering generation of cotton bollworm appear to have significantly contributed to the suppression of cotton bollworm observed throughout northern China in the past decade.