Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1

• Published in: Journal of virological methods 2011, Vol.171 (1), p.74-80
• Main Authors: Jiang, Tao, Liang, Zhong, Ren, Weiwei, Chen, Juan, Zhi, Xiaoying, Qi, Guangyu, Yang, Yamin, Liu, Zaixing, Liu, Xiangtao, Cai, Xuepeng
• Format: Article
• Language: English
• Subjects: Animals; antibodies; Antibodies, Viral; Biological and medical sciences; China; Colloidal gold particles; color; diagnostic sensitivity; enzyme-linked immunosorbent assay; foot-and-mouth disease; Foot-and-mouth disease virus; Foot-and-Mouth Disease Virus - classification; Foot-and-Mouth Disease Virus - immunology; Foot-and-Mouth Disease Virus - isolation & purification; Foot-and-mouth disease virus o; Fundamental and applied biological sciences. Psychology; gold; Gold Colloid; Guinea Pigs; immunoaffinity chromatography; Immunoassay - methods; Lateral flow immunoassay; Mice; Microbiology; Rabbits; Reagent Kits, Diagnostic; Sensitivity and Specificity; serotypes; Serotyping; Techniques used in virology; Virology; Virology - methods; viruses
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2010.10.002

A lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes O, A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples ( n = 396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples ( n = 95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%.