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Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles
Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-lik...
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| Published in: | Virus research 2011-02, Vol.155 (2), p.514-519 |
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| cites | cdi_FETCH-LOGICAL-c489t-db4e7ba6aa55b71d6cd06b4c0049681b34a58807ff45459295fec99e7a6cc8123 |
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| creator | Zielonka, Anja Verschoor, Ernst J. Gedvilaite, Alma Roesler, Uwe Müller, Hermann Johne, Reimar |
| description | Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-like particles (VLPs) with a diameter of approximately 45
nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans. |
| doi_str_mv | 10.1016/j.virusres.2010.12.009 |
| format | article |
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nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2010.12.009</identifier><identifier>PMID: 21187117</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Wild ; antibodies ; Antibodies, Viral - blood ; Ape Diseases - blood ; Ape Diseases - immunology ; Capsid Proteins - genetics ; Capsid Proteins - immunology ; Capsid Proteins - metabolism ; Chimpanzee ; coat proteins ; Cross Reactions - immunology ; ELISA ; enzyme-linked immunosorbent assay ; erythrocytes ; feces ; Haemagglutination ; humans ; immunoblotting ; Pan troglodytes ; Pan troglodytes - immunology ; pathogenicity ; polymerase chain reaction ; Polyomavirus ; Polyomavirus - immunology ; Polyomavirus Infections - blood ; Polyomavirus Infections - immunology ; Polyomavirus Infections - veterinary ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae - genetics ; screening ; Simian virus 40 - chemistry ; Simian virus 40 - immunology ; Virion - chemistry ; Virion - immunology ; Virion - ultrastructure ; Virus-like particles ; yeasts</subject><ispartof>Virus research, 2011-02, Vol.155 (2), p.514-519</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-db4e7ba6aa55b71d6cd06b4c0049681b34a58807ff45459295fec99e7a6cc8123</citedby><cites>FETCH-LOGICAL-c489t-db4e7ba6aa55b71d6cd06b4c0049681b34a58807ff45459295fec99e7a6cc8123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21187117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zielonka, Anja</creatorcontrib><creatorcontrib>Verschoor, Ernst J.</creatorcontrib><creatorcontrib>Gedvilaite, Alma</creatorcontrib><creatorcontrib>Roesler, Uwe</creatorcontrib><creatorcontrib>Müller, Hermann</creatorcontrib><creatorcontrib>Johne, Reimar</creatorcontrib><title>Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-like particles (VLPs) with a diameter of approximately 45
nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans.</description><subject>Animals</subject><subject>Animals, Wild</subject><subject>antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Ape Diseases - blood</subject><subject>Ape Diseases - immunology</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - immunology</subject><subject>Capsid Proteins - metabolism</subject><subject>Chimpanzee</subject><subject>coat proteins</subject><subject>Cross Reactions - immunology</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>erythrocytes</subject><subject>feces</subject><subject>Haemagglutination</subject><subject>humans</subject><subject>immunoblotting</subject><subject>Pan troglodytes</subject><subject>Pan troglodytes - immunology</subject><subject>pathogenicity</subject><subject>polymerase chain reaction</subject><subject>Polyomavirus</subject><subject>Polyomavirus - immunology</subject><subject>Polyomavirus Infections - blood</subject><subject>Polyomavirus Infections - immunology</subject><subject>Polyomavirus Infections - veterinary</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>screening</subject><subject>Simian virus 40 - chemistry</subject><subject>Simian virus 40 - immunology</subject><subject>Virion - chemistry</subject><subject>Virion - immunology</subject><subject>Virion - ultrastructure</subject><subject>Virus-like particles</subject><subject>yeasts</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAURi0EokPhFYp3rDLYjmMnO1DLn1SJBXRtOfbN9A5JHOxkYHgEnhoPaRE7WFm6Ovf7rnwIueBsyxlXL_fbA8YlRUhbwU5DsWWseUA2vNai0LIRD8kmg3XBNRNn5ElKe8aYKrV6TM4EzxjnekN-XsEMbsYw0tBRd4vDZMcfAHQK_TEM9ndLkSZw2KGjdpyxDR4hURyps9OMB8hTT79h7wtnl93t_FdMokvCcUePYNNcwPcpH5zA0zW2xy-5yMYZXQ_pKXnU2T7Bs7v3nNy8ffP58n1x_fHdh8vX14WTdTMXvpWgW6usrapWc6-cZ6qVjjHZqJq3pbRVXTPddbKSVSOaqgPXNKCtcq7mojwnL9bcKYavC6TZDJgc9L0dISzJ1IqVumHsP0ipZVmqSmVSraSLIWUrnZkiDjYeDWfmJMzszb0wcxJmuDBZWF68uKtY2gH8n7V7Qxl4vgKdDcbuIiZz8yknqGxT1ExUmXi1EpA_7YAQTXIIowOPMas1PuC_rvgFC_G36A</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Zielonka, Anja</creator><creator>Verschoor, Ernst J.</creator><creator>Gedvilaite, Alma</creator><creator>Roesler, Uwe</creator><creator>Müller, Hermann</creator><creator>Johne, Reimar</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>20110201</creationdate><title>Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles</title><author>Zielonka, Anja ; Verschoor, Ernst J. ; Gedvilaite, Alma ; Roesler, Uwe ; Müller, Hermann ; Johne, Reimar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-db4e7ba6aa55b71d6cd06b4c0049681b34a58807ff45459295fec99e7a6cc8123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Animals, Wild</topic><topic>antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Ape Diseases - blood</topic><topic>Ape Diseases - immunology</topic><topic>Capsid Proteins - genetics</topic><topic>Capsid Proteins - immunology</topic><topic>Capsid Proteins - metabolism</topic><topic>Chimpanzee</topic><topic>coat proteins</topic><topic>Cross Reactions - immunology</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>erythrocytes</topic><topic>feces</topic><topic>Haemagglutination</topic><topic>humans</topic><topic>immunoblotting</topic><topic>Pan troglodytes</topic><topic>Pan troglodytes - immunology</topic><topic>pathogenicity</topic><topic>polymerase chain reaction</topic><topic>Polyomavirus</topic><topic>Polyomavirus - immunology</topic><topic>Polyomavirus Infections - blood</topic><topic>Polyomavirus Infections - immunology</topic><topic>Polyomavirus Infections - veterinary</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>screening</topic><topic>Simian virus 40 - chemistry</topic><topic>Simian virus 40 - immunology</topic><topic>Virion - chemistry</topic><topic>Virion - immunology</topic><topic>Virion - ultrastructure</topic><topic>Virus-like particles</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zielonka, Anja</creatorcontrib><creatorcontrib>Verschoor, Ernst J.</creatorcontrib><creatorcontrib>Gedvilaite, Alma</creatorcontrib><creatorcontrib>Roesler, Uwe</creatorcontrib><creatorcontrib>Müller, Hermann</creatorcontrib><creatorcontrib>Johne, Reimar</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zielonka, Anja</au><au>Verschoor, Ernst J.</au><au>Gedvilaite, Alma</au><au>Roesler, Uwe</au><au>Müller, Hermann</au><au>Johne, Reimar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>155</volume><issue>2</issue><spage>514</spage><epage>519</epage><pages>514-519</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-like particles (VLPs) with a diameter of approximately 45
nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21187117</pmid><doi>10.1016/j.virusres.2010.12.009</doi><tpages>6</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection |
| subjects | Animals Animals, Wild antibodies Antibodies, Viral - blood Ape Diseases - blood Ape Diseases - immunology Capsid Proteins - genetics Capsid Proteins - immunology Capsid Proteins - metabolism Chimpanzee coat proteins Cross Reactions - immunology ELISA enzyme-linked immunosorbent assay erythrocytes feces Haemagglutination humans immunoblotting Pan troglodytes Pan troglodytes - immunology pathogenicity polymerase chain reaction Polyomavirus Polyomavirus - immunology Polyomavirus Infections - blood Polyomavirus Infections - immunology Polyomavirus Infections - veterinary Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - genetics screening Simian virus 40 - chemistry Simian virus 40 - immunology Virion - chemistry Virion - immunology Virion - ultrastructure Virus-like particles yeasts |
| title | Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles |
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