cDNA Expression Analysis of a Human Radiosensitive-Radioresistant Cell Line Model Identifies Telomere Function as a Hallmark of Radioresistance

To achieve a more complete understanding of the molecular mechanisms underlying tumor radioresistance, we established a radioresistant cell line from the human larynx squamous cell carcinoma cell line Hep-2 after long-term radiation induction. The biological features of the resulting cell lines were...

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Bibliographic Details
Published in:Radiation research 2010-11, Vol.174 (5), p.550-557
Main Authors: Zhou, Fu-Xiang, Xiong, Jie, Luo, Zhi-Guo, Dai, Jing, Yu, Hai-Jun, Liao, Zheng-Kai, Lei, Han, Xie, Cong-Hua, Zhou, Yun-Feng
Format: Article
Language:English
Subjects:
Apoptosis
B lymphocytes
Cell cycle
Cell Cycle - genetics
Cell Cycle - radiation effects
Cell growth
Cell Line, Tumor
Cell lines
Cell Proliferation - radiation effects
Complementary DNA
DNA
Gene Expression Profiling
Humans
Oligonucleotide Array Sequence Analysis
Radiation tolerance
Radiation Tolerance - genetics
Radiation Tolerance - radiation effects
REGULAR ARTICLES
Space life sciences
Telomerase - metabolism
Telomere - metabolism
Telomere - radiation effects
Telomeres
Tumor cell line
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Summary:To achieve a more complete understanding of the molecular mechanisms underlying tumor radioresistance, we established a radioresistant cell line from the human larynx squamous cell carcinoma cell line Hep-2 after long-term radiation induction. The biological features of the resulting cell lines were characterized. cDNA microarray technology was used to measure the alterations of gene expression in the radioresistant cells. We found that certain genes associated with DNA repair, cell cycle, apoptosis, etc. were significantly changed. In particular, genes related to telomeres, such as POT1, were significantly altered. Radioresistant cells had higher telomerase activity and longer telomeres than their parental cells. Our research suggests that telomere function is a novel hallmark of cellular radiosensitivity, and the mechanistic link between telomere maintenance and radiosensitivity may involve the genes and pathways we implicated in this study.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR1657.1