Biogeochemical factors influencing net mercury methylation in contaminated freshwater sediments from the St. Lawrence River in Cornwall, Ontario, Canada

• Published in: The Science of the total environment 2011-02, Vol.409 (5), p.968-978
• Main Authors: Avramescu, Mary-Luyza, Yumvihoze, Emmanuel, Hintelmann, Holger, Ridal, Jeff, Fortin, Danielle, R.S. Lean, David
• Format: Article
• Language: English
• Subjects: Anaerobiosis; Applied sciences; Bacteria; Bacteria, Anaerobic - metabolism; Biological and physicochemical properties of pollutants. Interaction in the soil; Biotransformation; Demethylation; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Exact sciences and technology; FeRP; Geologic Sediments - chemistry; Geologic Sediments - microbiology; Geological Phenomena; Inhibitors; Iron; Mercury; Mercury - analysis; Mercury - chemistry; Mercury - metabolism; Methylation; Methylmercury; Methylmercury Compounds - analysis; Methylmercury Compounds - chemistry; Methylmercury Compounds - metabolism; Microorganisms; MPA; Ontario; Pollution; Pollution, environment geology; Reduction; Rivers - chemistry; Rivers - microbiology; Sediments; Soil and sediments pollution; SRB
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2010.11.016

The activity of various anaerobic microbes, including sulfate reducers (SRB), iron reducers (FeRP) and methanogens (MPA) has been linked to mercury methylation in aquatic systems, although the relative importance of each microbial group in the overall process is poorly understood in natural sediments. The present study focused on the biogeochemical factors (i.e. the relative importance of various groups of anaerobic microbes (FeRP, SRB, and MPA) that affect net monomethylmercury (MMHg) formation in contaminated sediments of the St. Lawrence River (SRL) near Cornwall (Zone 1), Ontario, Canada. Methylation and demethylation potentials were measured separately by using isotope-enriched mercury species ( 200Hg 2+ and MM 199Hg +) in sediment microcosms treated with specific microbial inhibitors. Sediments were sampled and incubated in the dark at room temperature in an anaerobic chamber for 96 h. The potential methylation rate constants (K m) and demethylation rates (K d) were found to differ significantly between microcosms. The MPA-inhibited microcosm had the highest potential methylation rate constant (0.016 d −1), whereas the two SRB-inhibited microcosms had comparable potential methylation rate constants (0.003 d −1 and 0.002 d −1, respectively). The inhibition of methanogens stimulated net methylation by inhibiting demethylationand by stimulating methylation along with SRB activity. The inhibition of both methanogens and SRB was found to enhance the iron reduction rates but did not completely stop MMHg production. The strong positive correlation between K m and Sulfate Reduction Rates (SRR) and between K d and Methane Production Rates (MPR) supports the involvement of SRB in Hg methylation and MPA in MMHg demethylation in the sediments. In contrast, the strong negative correlation between K d and Iron Reduction Rates (FeRR) shows that the increase in FeRR corresponds to a decrease in demethylation, indicating that iron reduction may influence net methylation in the SLR sediments by decreasing demethylation rather than favouring methylation. ► Investigation of methylmercury formation and demethylation in contaminated sediments. ► Use of enriched Hg isotopes and specific bacterial inhibitors. ► The inhibition of methanogens stimulated net methylation. ► The inhibition of methanogens and SRB enhanced iron reduction ► Increased iron reduction corresponded to a decrease in demethylation.