Enzyme based assays in a sequential injection format: A review

Sequential injection analysis systems have been extensively exploited in the last decades for the implementation of enzyme based assays aiming the evaluation of enzyme activity or the determination of specific analytes. The most prominent aspects of the automation of enzymatic assays in these system...

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Bibliographic Details
Published in:Analytica chimica acta 2011-03, Vol.689 (2), p.160-177
Main Authors: Silvestre, Cristina I.C., Pinto, Paula C.A.G., Segundo, Marcela A., Saraiva, M. Lúcia M.F.S., Lima, José L.F.C.
Format: Article
Language:English
Subjects:
Analytical chemistry
Assaying
Automation
Beads
Biocatalysis
Chemistry
Enzymatic reactor
Enzyme
Enzyme activity
Enzyme Assays - methods
Enzymes
Enzymes - metabolism
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Exact sciences and technology
Flow Injection Analysis - methods
Focusing
Immobilized enzyme
Mathematical analysis
Miniaturization
Miscellaneous
Platforms
Sequential injection analysis
Spectrometric and optical methods
Strategy
Valves
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Summary:Sequential injection analysis systems have been extensively exploited in the last decades for the implementation of enzyme based assays aiming the evaluation of enzyme activity or the determination of specific analytes. The most prominent aspects of the automation of enzymatic assays in these systems are discussed in this review. Special attention is devoted to the mode of enzyme manipulation in homogeneous or heterogeneous media and to the comparison with batch and flow injection enzyme methodologies. The possibility of implementing strategies for the enhancement of selectivity in specific determinations is also addressed. The more recent trends in this field are discussed focusing mainly on the miniaturization resorting to the lab-on valve platform as well as on the bead injection concept.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2011.01.048