Vaccine efficacy of Mycobacterium bovis BCG against Mycobacterium sp. infection in amberjack Seriola dumerili

Mycobacteriosis, caused by the intracellular parasitism Mycobacterium sp., causes economic damages to aquaculture production in Japan, particularly in seriola fish production. Antibiotics are not effective against Mycobacterium sp. and so a potent vaccine is needed. We previously reported that BCG v...

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Bibliographic Details
Published in:Fish & shellfish immunology 2011-02, Vol.30 (2), p.467-472
Main Authors: Kato, Goshi, Kato, Keitaro, Saito, Kei, Pe, Yo, Kondo, Hidehiro, Aoki, Takashi, Hirono, Ikuo
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - immunology
adaptive immunity
Amberjack
Animals
antibiotics
antigens
bacterial infections
Bacterial Load
BCG vaccine
BCG Vaccine - immunology
Fish Diseases - mortality
Fish Diseases - prevention & control
fish production
flounder
injection site
Live attenuated vaccine
M. bovis BCG
major genes
Marine
mortality
muscles
Mycobacteriosis
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium bovis
Mycobacterium bovis BCG
Mycobacterium Infections - mortality
Mycobacterium Infections - prevention & control
Mycobacterium Infections - veterinary
Mycobacterium ulcerans
Paralichthys olivaceus
parasitism
Perciformes
Phylogeny
plate count
Pleuronectiformes
Seriola
Seriola dumerili
spleen
Spleen - microbiology
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Summary:Mycobacteriosis, caused by the intracellular parasitism Mycobacterium sp., causes economic damages to aquaculture production in Japan, particularly in seriola fish production. Antibiotics are not effective against Mycobacterium sp. and so a potent vaccine is needed. We previously reported that BCG vaccine ( Mycobacterium bovis BCG) induces adaptive immunity against Mycobacterium sp. in Japanese flounder, Paralichthys olivaceus. In a phylogenetic tree, the genes for a major antigen, the Ag85 complex, in Mycobacterium sp. TUMSAT-Msp001 are closely related to homologues in Mycobacterium ulcerans. M. bovis BCG was detected until 7 days post-injection at the injection site (muscle) and 28 days post-vaccination in spleen. Cumulative mortality of amberjack, Seriola dumerili vaccinated intramuscularly (i.m.) and intraperitoneally (i.p.) with M. bovis BCG was 32.3% and 59.5% respectively, at 24 days post-infection of Mycobacterium sp., compared to 97.8% in PBS-injected fish. The bacterial counts of Mycobacterium sp. in spleen of both i.m.-and i.p.-vaccinated fish (6.2 × 10 3 and 1.3 × 10 4 CFU/mg tissue, respectively) at 20 days post-infection were significantly lower ( P 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2010.11.002