In vitro antioxidant and inhibitory potential of Terminalia bellerica and Emblica officinalis fruits against LDL oxidation and key enzymes linked to type 2 diabetes

The present study evaluated the free radical scavenging capacity and antioxidant potential of different solvent extracts (Hexane (HE), ethyl acetate (EA), methanol (ME), 70% methanol (MW) and Water (WA)) of Terminalia bellerica (TB) and Emblica officinalis (EB) fruits. Methanol extract (ME) of TB an...

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Bibliographic Details
Published in:Food and chemical toxicology 2011-01, Vol.49 (1), p.125-131
Main Authors: Nampoothiri, Suresh V., Prathapan, A., Cherian, Ozhathil Lijo, Raghu, K.G., Venugopalan, V.V., Sundaresan, A.
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Biological and medical sciences
C2C12 cell lines
Cell Line
Chromatography, Liquid
Diabetes
Diabetes Mellitus, Type 2 - enzymology
Diabetes Mellitus, Type 2 - metabolism
Diabetes. Impaired glucose tolerance
Emblica officinalis
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Flow Cytometry
Free radical
In Vitro Techniques
LC–MS
LDL oxidation
Lipoproteins, LDL - metabolism
Mass Spectrometry
Medical sciences
Mice
Oxidation-Reduction
Oxidative Stress
Phyllanthus emblica - chemistry
Plant Extracts - pharmacology
Terminalia - chemistry
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Summary:The present study evaluated the free radical scavenging capacity and antioxidant potential of different solvent extracts (Hexane (HE), ethyl acetate (EA), methanol (ME), 70% methanol (MW) and Water (WA)) of Terminalia bellerica (TB) and Emblica officinalis (EB) fruits. Methanol extract (ME) of TB and EB fruits exhibited maximum scavenging activity against DPPH, superoxide, hydroxyl and nitric oxide radicals. Cell based antioxidant activity was assayed by flow cytometry using DCFH-DA as probe. Methanol extracts were also screened for their antidiabetic activity via inhibition of α-amylase, α-glucosidase and antiglycation assays. Results showed that ME of TB and EB can act as potent α-amylase and α-glucosidase inhibitor. Significant antiglycation activity also confirms the therapeutic potential of these extracts against diabetes. Both the extracts significantly inhibited the oxidation of LDL under in vitro conditions. Liquid chromatography–mass spectroscopy (LC–MS) analysis revealed that methanol extract of TB and EB contains ellagic acid and ascorbic acid as the major compound respectively.
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2010.10.006