Catechin-7-O- beta -D-Glucopyranoside Scavenges Free Radicals and Protects Human B Lymphoma BJAB Cells on H sub(2)O sub(2)-mediated Oxidative Stress

• Published in: Food science and biotechnology 2011-02, Vol.20 (1), p.151-158
• Main Authors: Baek, J-A, Son, Y-O, Fang, M, Lee, Y J, Cho, H-K, Whang, W K, Lee, J-C
• Format: Article
• Language: English
• Subjects: Phaseolus
• ISSN: 1226-7708
• DOI: 10.1007/s10068-011-0021-x

Catechin-7-O- beta -D-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H sub(2)O sub(2)). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H sub(2)O sub(2)-exposed cells was also investigated. CA-G treatment prevented H sub(2)O sub(2)-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H sub(2)O sub(2)-exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H sub(2)O sub(2). Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H sub(2)O sub(2)-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H sub(2)O sub(2) via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-G-rich red bean on the protection from oxidative damage.