Evaluation of bacterial strains isolated from oil-contaminated soil for production of polyhydroxyalkanoic acids (PHA)

Samples of eight geographically distinct soils contaminated with crude oil were screened for polyhydroxyalkanoic acid (PHA) producing bacterial strains. Twenty three bacterial strains were able to accumulate PHA when sodium gluconate or sodium octanoate was used as the sole carbon source. Biochemica...

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Bibliographic Details
Published in:Pedobiologia 2010-12, Vol.54 (1), p.25-30
Main Authors: Dalal, Jyotsana, Sarma, Priyangshu M., Lavania, Meeta, Mandal, Ajoy K., Lal, Banwari
Format: Article
Language:English
Subjects:
Acinetobacter
Bacteria
Burkholderia
Caulobacter
Oil-contaminated soil
Polyhydroxyalkanoic acids
Pseudomonas
Ralstonia
Sphingobacterium
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Summary:Samples of eight geographically distinct soils contaminated with crude oil were screened for polyhydroxyalkanoic acid (PHA) producing bacterial strains. Twenty three bacterial strains were able to accumulate PHA when sodium gluconate or sodium octanoate was used as the sole carbon source. Biochemical tests and 16S rRNA sequencing identified bacteria of the genera Pseudomonas, Acinetobacter, Sphingobacterium, Brochothrix, Caulobacter, Ralstonia, Burkholderia and Yokenella. Three of the bacterial strains have never been reported to produce PHA. The phylogenetic analysis of the PHA synthase ( phaC) gene of these bacteria showed a close homology with the phaC gene of different Pseudomonas species. This study indicates that stressed environments like oil-contaminated sites can be potential sources of medium-chain-length PHA producers.
ISSN:0031-4056
1873-1511
DOI:10.1016/j.pedobi.2010.08.004