In vivo evidence of enhanced di-methylation of histone H3 K4 on upregulated genes in adipose tissue of diabetic db/db mice

► Identification of upregulated genes in adipose tissue of diabetic db/db mice. ► Higher di-methylation of histone H3 K4 on the upregulated genes. ► First report of histone modification on genes in adipose tissues of diabetic animals. Di-methylation of histone H3 lysine (K) 4, a component of the epi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2011-01, Vol.404 (1), p.223-227
Main Authors: Fujimoto, Saki, Goda, Toshinao, Mochizuki, Kazuki
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - metabolism
Animals
chromatin
Di-methylation
Diabetes
Diabetes Mellitus - genetics
Diabetes Mellitus - metabolism
Epigenesis, Genetic
epigenetics
Gene Expression Profiling
genes
Histone H3 K4
histones
Histones - metabolism
lysine
Mesentery - metabolism
Methylation
Mice
Mice, Inbred Strains
microarray technology
Oligonucleotide Array Sequence Analysis
precipitin tests
Transcriptional Activation
Up-Regulation
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Summary:► Identification of upregulated genes in adipose tissue of diabetic db/db mice. ► Higher di-methylation of histone H3 K4 on the upregulated genes. ► First report of histone modification on genes in adipose tissues of diabetic animals. Di-methylation of histone H3 lysine (K) 4, a component of the epigenetic memory, is associated with gene transactivation. In this study, we examined whether the development of diabetes induces di-methylation of histone H3 K4 on the upregulated genes. We searched for upregulated genes in mesenteric adipose tissue of insulin-resistant/diabetic db/db mice compared with non-diabetic db/m mice using microarray analysis. We also performed chromatin immunoprecipitation assays for di-methylation of histone H3 K4 in the upregulated genes in mesenteric adipose tissue of db/m and db/db mice. Di-methylation of histone H3 K4 was enhanced at the upstream and/or transcribed regions of upregulated genes including Atp6v0d2, Mmp12, Trem2 and Clec4d genes in mesenteric adipose tissue of db/db mice, as compared with db/m mice. These results suggest that di-methylation of histone H3 K4 is involved in the induction of Atp6v0d2, Mmp12, Trem2 and Clec4d in mesenteric adipose tissue in db/db mice.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.11.097