Effect of environmental factors and carbohydrate on gellan gum production

Submerged culture fermentation studies were carried out in batch mode for optimizing the environmental parameters and carbon source requirement by Pseudomonas elodea for the production of gellan gum. The maximum production of gellan gum was obtained with 16-h-old culture and 8% inoculum at 30 degree...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2002-07, Vol.102 (1-6), p.129-140
Main Authors: Kanari, B, Banik, R.R, Upadhyay, S.N
Format: Article
Language:English
Subjects:
Bacteria
Carbohydrate Metabolism
Carbohydrates
Carbohydrates - pharmacology
Carbon
Carbon sources
Chemical Precipitation
Colorimetry
Culture Media
Elodea
Environmental effects
Environmental factors
Fermentation
Gellan gum
Glucose
Hydrogen-Ion Concentration
Inoculum
isopropyl alcohol
Optimization
Polysaccharides, Bacterial - analysis
Polysaccharides, Bacterial - biosynthesis
Pseudomonas
Pseudomonas - growth & development
Pseudomonas - metabolism
Recovery
Solvents
Solvents - chemistry
Sphingomonas elodea
Starch
Studies
Sucrose
Temperature
Tetrahydrofuran
Time Factors
Viscosity
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Summary:Submerged culture fermentation studies were carried out in batch mode for optimizing the environmental parameters and carbon source requirement by Pseudomonas elodea for the production of gellan gum. The maximum production of gellan gum was obtained with 16-h-old culture and 8% inoculum at 30 degrees C and pH 7.0 after 52 h of incubation (6.0 g/L). Of the various carbon sources tested, 2% sucrose, glucose, and soluble starch yielded considerably high amounts of gellan. Studies on the concentration of various carbohydrates on gellan gum production indicated that the optimum concentration of glucose and starch was 3%, whereas for sucrose it was 4%. The addition of glucose in the medium above 3% had a detrimental effect on gellan yield. The investigation of intermediate two-step addition of glucose under identical conditions of fermentation showed an enhanced production of gellan (8.12 g/L) as compared with the control (6.0 g/L). To optimize the recovery of gellan from fermented broth, different solvents were tested for precipitation of gellan gum. Among the various solvents tested, tetrahydrofuran gave better recovery of gellan (82%) as compared with the conventional solvent isopropanol (49%).
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/abab:102-103:1-6:129