Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs

Digital gene expression (DGE) analysis offers a route to gene discovery which by‐passes the need to develop bespoke arrays for nonmodel species, and is therefore a potentially valuable tool for molecular ecologists. Scottish blackface sheep, which vary in resistance to the common abomasal parasitic...

Full description

Saved in:
Bibliographic Details
Published in:Molecular ecology 2011-03, Vol.20 (5), p.910-919
Main Authors: PEMBERTON, J. M., BERALDI, D., CRAIG, B. H., HOPKINS, J.
Format: Article
Language:English
Subjects:
agriculture
Animal diseases
Animal populations
Animals
bioinformatics/phyloinformatics
disease biology
Female
Gastrointestinal Tract - immunology
Gastrointestinal Tract - parasitology
Gene expression
Gene Expression Profiling
genomics/proteomics
Immune system
Immunity, Innate - genetics
Nematoda
Parasitic diseases
Sheep
Sheep Diseases - genetics
Sheep Diseases - immunology
Sheep Diseases - parasitology
Sheep, Domestic - genetics
Sheep, Domestic - immunology
Sheep, Domestic - parasitology
transcriptomics
Trichostrongyloidea - physiology
Trichostrongyloidiasis - genetics
Trichostrongyloidiasis - immunology
Trichostrongyloidiasis - veterinary
worms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Digital gene expression (DGE) analysis offers a route to gene discovery which by‐passes the need to develop bespoke arrays for nonmodel species, and is therefore a potentially valuable tool for molecular ecologists. Scottish blackface sheep, which vary in resistance to the common abomasal parasitic nematode Teladorsagia circumcincta, were trickle‐infected with L3 larvae over 3 months to mimic the natural progression of infection. DGE was performed on abomasal lymph node tissue after the resolution of infection in resistant animals. Susceptible (low resistance) animals showed a large number of differentially expressed genes associated with inflammation and cell activation, but generally few differentially regulated genes in either the susceptible or the resistant group were directly involved in the adaptive immune function. Our results are consistent with the hypothesis that both resistance and susceptibility are active responses to infection and that susceptibility is associated with dysfunction in T cell differentiation and regulation.
ISSN:0962-1083
1365-294X
DOI:10.1111/j.1365-294X.2010.04992.x