Extracellular proteolytic activity of bacteria from soda-salt lakes of Transbaikalia

Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not res...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and microbiology 2010-11, Vol.46 (6), p.580-585
Main Authors: Lavrenteva, E. V, Dunaevsky, Ya. E, Kozyreva, L. P, Radnagurueva, A. A, Namsaraev, B. B
Format: Article
Language:English
Subjects:
Alkalinity
Bacteria
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Enzymatic activity
Freshwater
Life Sciences
Medical Microbiology
Microbiology
Nitrogen
Peptones
Salt lakes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not result in increase of enzyme activity in cultural liquid. It was indicated that secreting proteinases of the studied bacteria strains possess narrow substrate specificity, hydrolyze proteins and n-nitroanilide substrates have maximal activity during GlpAALpNA hydrolysis. Data of inhibitory analysis and substrate specificity of studied extracellular enzymes indicate that they belong to a class of serine proteinases of subtilisin-like type.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683810060049