Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (Characiformes, Prochilodontidae) obtained by DNA probes

In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (gen...

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Bibliographic Details
Published in:Genetica 2010-12, Vol.138 (11-12), p.1133-1139
Main Authors: Voltolin, Tatiana Aparecida, Laudicina, Alejandro, Senhorini, José Augusto, Bortolozzi, Jehud, Oliveira, Cláudio, Foresti, Fausto, Porto-Foresti, Fábio
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
B-chromosome
Biomedical and Life Sciences
Characiformes
Chromatin
Chromosomes
Cytogenetic Analysis
Deoxyribonucleic acid
DNA
DNA Probes - genetics
evolution
Evolution, Molecular
Evolutionary Biology
Female
Fishes - genetics
Freshwater
Genomics
Human Genetics
In Situ Hybridization, Fluorescence
Karyotyping
Life Sciences
Male
Microbial Genetics and Genomics
Molecular organization
Origin
Original Research
Plant Genetics and Genomics
Probes
Prochilodontidae
Prochilodus lineatus
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Summary:In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (genomic probe) from an individual without B-chromosome. The specific B-chromosome was scraped and processed to obtain DNA with amplification by DOP-PCR, and so did the genomic probe DNA. Fluorescence in situ hybridization using the B-chromosome probe labeled with dUTP-Tetramethyl-rhodamine and the genomic probe labeled with digoxigenin-FITC permitted to establish that in this species supernumerary chromosomes with varying number and morphology had different structure of chromatin when compared to that of the regular chromosomes or A complement, since only these extra elements were labeled in the metaphases. The present findings suggest that modifications in the chromatin structure of B-chromosomes to differentiate them from the A chromosomes could occur along their dispersion in the individuals of the population.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-010-9502-8