Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines

Viral nervous necrosis (VNN), also known as viral encephalopathy and retinopathy (VER), is an emerging disease affecting larvae and juveniles of many farmed marine fish species in Asia, Australia,Europe and North America. Mass mortality occurred in 14-day old larval sea bass Lates calcarifer at a ha...

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Bibliographic Details
Published in:JARQ. Japan agricultural research quarterly 2004/01/30, Vol.38(1), pp.69-73
Main Authors: Maeno, Y. (Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki), De La Pena, L.D, Cruz-Lacierda, E.R
Format: Article
Language:English
Subjects:
Dicentrarchus labrax
FISH DISEASES
LARVAE
LATES CALCARIFER
Marine
NECROSIS
NERVES
Nodavirus
PARTICLE SIZE
PATHOGENS
PHILIPPINES
piscine nodavirus
VER
VIROSES
VIRUSES
VNN
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Summary:Viral nervous necrosis (VNN), also known as viral encephalopathy and retinopathy (VER), is an emerging disease affecting larvae and juveniles of many farmed marine fish species in Asia, Australia,Europe and North America. Mass mortality occurred in 14-day old larval sea bass Lates calcarifer at a hatchery in the Philippines associated with clinical signs such as abnormal swimming behavior and pale-gray discoloration of the body. Histological investigations in moribund fish revealed marked vacuolation in the retina and brain. Cytopathic effects (CPE) were observed in SSN-1 cells inoculated with the tissue filtrate of affected sea bass. A piscine nodavirus, the causative agent of VNN, was detected in the affected tissues and SSN-1 cells inoculated with the tissue filtrate of affected fish by RT-PCR. Electron microscopy revealed non-enveloped viral particles, 22-28 nm in diameter, in the cytoplasm of the brain and retina of affected fish and in the cytoplasm of VNN-infected SSN-1 cells after CPE appeared. These results indicate that mass mortality of sea bass larvae in the Philippines was caused by a piscine nodavirus.
ISSN:0021-3551
2185-8896
DOI:10.6090/jarq.38.69