Evaluation of Cell Recycle on Thermomyces lanuginosus Xylanase A Production by Pichia pastoris GS 115

This work aims to evaluate cell recycle of a recombinant strain of Pichia pastoris GS115 on the Xylanase A (XynA) production of Thermomyces lanuginosus IOC-4145 in submerged fermentation. Fed-batch processes were carried out with methanol feeding at each 12 h and recycling cell at 24, 48, and 72 h....

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Published in:Applied biochemistry and biotechnology 2006, Vol.129 (1-3), p.226-233
Main Authors: Ferreira, V, Nolasco, P.C, Castro, A.M, Silva, J.N.C, Santos, A.S, Damaso, M.C.T, Pereira, N. Jr
Format: Article
Language:English
Subjects:
Ascomycota - enzymology
Ascomycota - genetics
Biochemistry
biological production
Bioreactors - microbiology
Cell Culture Techniques - methods
Cell Proliferation
Cell Survival
Cells
Endo-1,4-beta Xylanases - biosynthesis
Endo-1,4-beta Xylanases - genetics
Enzymatic activity
enzyme production
Enzymes
Fermentation
Pichia - physiology
Pichia pastoris
Protein Engineering - methods
Studies
Thermomyces lanuginosus
xylanases
Yeast
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Summary:This work aims to evaluate cell recycle of a recombinant strain of Pichia pastoris GS115 on the Xylanase A (XynA) production of Thermomyces lanuginosus IOC-4145 in submerged fermentation. Fed-batch processes were carried out with methanol feeding at each 12 h and recycling cell at 24, 48, and 72 h. Additionally, the influence of the initial cell concentration was investigated. XynA production was not decreased with the recycling time, during four cell recycles, using an initial cell concentration of 2.5 g/L. The maximum activity was 14,050 U/L obtained in 24 h of expression. However, when the initial cell concentration of 0.25 g/L was investigated, the enzymatic activity was reduced by 30 and 75% after the third and fourth cycles, respectively. Finally, it could be concluded that the initial cell concentration influenced the process performance and the interval of cell recycle affected enzymatic production.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:129:1:226