comparison of in vivo antigenotoxic and antioxidative capacity of two propylene glycol extracts of Calendula officinalis (marigold) and vitamin E in young growing pigs

The objective of the study was to evaluate the protective effect of Calendula officinalis propylene glycol extracts against oxidative DNA damage and lipid peroxidation induced by high polyunsaturated fatty acid (PUFA) intake in young growing pigs. Forty young growing pigs were assigned to five treat...

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Published in:Journal of animal physiology and animal nutrition 2009-12, Vol.93 (6), p.688-694
Main Authors: Frankič, T, Salobir, K, Salobir, J
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animal Nutritional Physiological Phenomena
Animals
antioxidant
Antioxidants - chemistry
Antioxidants - pharmacology
Calendula - chemistry
Calendula officinalis
Diet - veterinary
DNA damage
DNA Damage - drug effects
lipid peroxidation
Lipid Peroxidation - drug effects
oxidative stress
Plant Extracts - chemistry
Plant Extracts - pharmacology
Propylene Glycol - chemistry
Swine - growth & development
Vitamin E - chemistry
Vitamin E - pharmacology
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Summary:The objective of the study was to evaluate the protective effect of Calendula officinalis propylene glycol extracts against oxidative DNA damage and lipid peroxidation induced by high polyunsaturated fatty acid (PUFA) intake in young growing pigs. Forty young growing pigs were assigned to five treatment groups: control; oil (linseed oil supplementation); C. officinalis 1 and 2 groups (linseed oil plus 3 ml/day of C. officinalis propylene glycol extracts); and vitamin E group (linseed oil plus 100 mg/kg of vitamin E). Lymphocyte DNA fragmentation and 24-h urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) excretion were measured to determine DNA damage. Lipid peroxidation was studied by analysing plasma and urine malondialdehyde (MDA), and urine isoprostane concentrations (iPF2α-VI), total antioxidant status of plasma and glutathione peroxidase (GPx) assays. C. officinalis 1 (extract from petals) effectively protected DNA from oxidative damage. It indicated a numerical trend towards the reduction of plasma MDA and urinary iPF2α-VI excretion. Its effect was comparable with that of vitamin E. C. officinalis 2 (extract from flower tops) showed less antioxidant potential than the extract from petals. We can conclude that the amount of C. officinalis extracts proposed for internal use by traditional medicine protects the organism against DNA damage induced by high PUFA intake.
ISSN:0931-2439
1439-0396
DOI:10.1111/j.1439-0396.2008.00855.x