Pollen donor composition during the early phases of reproduction revealed by DNA genotyping of pollen grains and seeds of Castanea crenata

In plants, pollen donor composition can differ during the early phases of reproduction through various selection mechanisms favouring self, related or nonrelated pollen donors, but such differences have not been examined under natural conditions because paternity is difficult to analyse in a natural...

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Bibliographic Details
Published in:The New phytologist 2009-06, Vol.182 (4), p.994-1002
Main Authors: Hasegawa, Yoichi, Suyama, Yoshihisa, Seiwa, Kenji
Format: Article
Language:English
Subjects:
Alleles
Castanea crenata
Chi-Square Distribution
Chromosome Segregation - genetics
DNA, Plant - genetics
Fagaceae
Fagaceae - genetics
Fagaceae - physiology
Flowers - genetics
genetic structure
Genotype
Geography
Heterozygote
Japan
mating system
Microsatellite Repeats - genetics
paternity analysis
Phylogeny
Pollen - genetics
pollen donor composition
Pollination - genetics
Reproduction - genetics
Seeds - genetics
self-pollination
single pollen grain
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Summary:In plants, pollen donor composition can differ during the early phases of reproduction through various selection mechanisms favouring self, related or nonrelated pollen donors, but such differences have not been examined under natural conditions because paternity is difficult to analyse in a natural setting. Here, we performed paternity analyses based on microsatellite genotyping of individual pollen grains deposited on female flowers (n = 773) and seeds (n = 304) to evaluate pollen donor composition from three individuals of the insect-pollinated monoecious tree Castanea crenata in a natural forest. Spatial genetic structure was also investigated. A mean self-pollen rate of 90.2% was observed at the pollination stage, but a low selfing rate of 0.3% was observed at the seed stage. In outcross events, however, pairwise distance and relatedness between maternal and paternal parents were not different between pollination and seed stages. We also observed significant positive relatedness, based on clear fine-scale genetic structure of individual trees within 80 m of one another, and 71% of seeds were derived using pollen grains of related trees within 80 m. The results suggest that the mechanism of self-incompatibility strongly avoids self-pollen before seed production. However, the avoidance of biparental inbreeding was not obvious between pollination and seed stages.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2009.02806.x