The NucleoSpin ® DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: An alternative to microdialysis filtration

AbstractTraditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon ® and Microcon ® centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using Nucl...

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Bibliographic Details
Published in:Forensic science international : genetics 2011-06, Vol.5 (3), p.226-230
Main Authors: Hudlow, William R, Krieger, Robert, Meusel, Markus, Sehhat, Joshua C, Timken, Mark D, Buoncristiani, Martin R
Format: Article
Language:English
Subjects:
DNA - isolation & purification
Filtration - methods
Humans
Microdialysis - methods
Microsatellite Repeats
Pathology
Polymerase Chain Reaction
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Summary:AbstractTraditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon ® and Microcon ® centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin ® XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin ® XS column is approximately 30 min vs. approximately 1.5 h with the Centricon ® YM-100 filter device.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2010.03.005