Differential spectral phase interferometry for wide dynamic range surface plasmon resonance biosensing

We introduce a novel wide dynamic range phase-sensitive surface plasmon resonance (SPR) biosensor based on differential spectral interferometry. Superseding conventional spectroscopic approach where only the SPR dip is monitored, our system acquires the spectral phase information of the entire elect...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors & bioelectronics 2010-12, Vol.26 (4), p.1593-1598
Main Authors: Ng, Siu-Pang, Wu, Chi-Man Lawrence, Wu, Shu-Yuen, Ho, Ho-Pui, Kong, S.K.
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Reactions
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensing Techniques - statistics & numerical data
Biosensor
Biosensors
Biotechnology
Cattle
Dielectric Spectroscopy - instrumentation
Dielectric Spectroscopy - methods
Differential spectral interferometry
Fundamental and applied biological sciences. Psychology
Gold
In Vitro Techniques
Interferometry - instrumentation
Interferometry - methods
Limit of Detection
Methods. Procedures. Technologies
Refractometry
Serum Albumin, Bovine - immunology
Serum Albumin, Bovine - metabolism
Surface plasmon resonance
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Surface Plasmon Resonance - statistics & numerical data
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We introduce a novel wide dynamic range phase-sensitive surface plasmon resonance (SPR) biosensor based on differential spectral interferometry. Superseding conventional spectroscopic approach where only the SPR dip is monitored, our system acquires the spectral phase information of the entire electromagnetic field that undergoes SPR transformation. Since the SPR-induced phase change is highly wavelength specific with fixed incident angle, ultra-high sensitivity achievable through phase-sensitive detection, as reported herein, is maintained continuously across the spectral domain in response to refractive index changes. Our system has demonstrated a detection limit of 2.2 × 10 −7 in terms of refractive index unit (RIU) using standard single-layer gold surface. In terms of biosensing performance, the estimated detection sensitivity obtained from bovine serum albumin (BSA) antibody–antigen binding experiments is 0.5 ng ml −1.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.07.128