Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube–inorganic composite membrane

We prepared silica nanotube with nanochannel-wall in the columnar pores of a commercial anodic alumina membrane (F127-MST), and then made up an artificial biomembrane consisting of a hierarchical structure by introducing an enzyme, formaldehyde dehydrogenase, into the silica nanochannels. The formal...

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Bibliographic Details
Published in:Journal of materials chemistry 2011-01, Vol.21 (1), p.251-256
Main Authors: Itoh, T., Shimomura, T., Hasegawa, Y., Mizuguchi, J., Hanaoka, T., Hayashi, A., Yamaguchi, A., Teramae, N., Ono, M., Mizukami, F.
Format: Article
Language:English
Subjects:
Electrodes
Enzymes
Formaldehyde
Membranes
Nanocomposites
Nanomaterials
Nanostructure
Silicon dioxide
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Summary:We prepared silica nanotube with nanochannel-wall in the columnar pores of a commercial anodic alumina membrane (F127-MST), and then made up an artificial biomembrane consisting of a hierarchical structure by introducing an enzyme, formaldehyde dehydrogenase, into the silica nanochannels. The formaldehyde dehydrogenase confined in membrane showed a very high activity for the reduction of NAD super(+) similar to that of the native, besides much higher electron transfer to the electrode than the native. At the same time, we clarified that the encapsulation of enzymes into the nanochannels enables enzymes to accumulate in high density without aggregation and be arranged regularly. These results indicate that F127-MST can provide a favorable method for enzyme immobilization on the electrode.
ISSN:0959-9428
1364-5501
DOI:10.1039/C0JM01523G