Determination of isometamidium residues in animal-derived foods by liquid chromatography–tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-04, Vol.879 (13), p.1008-1012
• Main Authors: Li, Y.G., Zou, Y., Zhang, L., Xi, C.X., Wang, G.M., Li, X.L., Zhang, J.Z., Li, Z.G.
• Format: Article
• Language: English
• Subjects: Acetonitrile; Analysis; Analytical, structural and metabolic biochemistry; Animal-derived food; Animals; Biological and medical sciences; Cattle; Chromatography; Chromatography, High Pressure Liquid - methods; detection limit; Drug Residues - analysis; Foods; Formates; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC–MS/MS; Isometamidium; Linear Models; liquid chromatography; Liquids; Liver; Mass spectrometry; Meat - analysis; Medical sciences; Methanol; milk; Milk - chemistry; Pharmacology. Drug treatments; Phenanthridines - analysis; Phenanthridines - chemistry; Reproducibility of Results; Residual detection; Residues; Sensitivity and Specificity; Standard deviation; tandem mass spectrometry; Tandem Mass Spectrometry - methods; tissues; Trypanocidal Agents - analysis; Trypanocidal Agents - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2011.02.040

In this paper, a new determination method for isometamidium residues in animal-derived foods was developed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Isometamidium residues in bovine tissues and milk were extracted with the mixed solution of acetonitrile and 0.25 mol/L of ammonium formate–methanol (v/v, 1:1), concentrated and degreased, and determined by LC–MS/MS with quantification by external standard method. The results showed that the peak area of chromatogram was linearly related to the concentration of isometamidium in the range of 1–100 μg/L, and the limits of detection (LOD) and quantification (LOQ) were 0.05 μg/kg and 5 μg/kg, respectively. The average recoveries of spiked samples were in the range of 73.8–93.9% with relative standard deviations ranged from 2.3% to 7.5%. This method is simple, accurate and suitable for the identification and quantification for isometamidium in animal-derived foods.