Direct-injection HPLC method of measuring micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-04, Vol.879 (13), p.1029-1032
• Main Authors: Uranishi, Hiroaki, Nakamura, Mitsuhiro, Nakamura, Hiroki, Ikeda, Yukari, Otsuka, Mayuko, Kato, Zenichiro, Tsuchiya, Teruo
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Calibration; Chromatography; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Direct injection; Dispersion hardening alloys; Echinocandins - blood; Emission; Fluorescence; Fluorescence detection; Fundamental and applied biological sciences. Psychology; General pharmacology; high performance liquid chromatography; Human; Humans; hydrophilicity; Hydrophobic and Hydrophilic Interactions; hydrophobicity; Interference; Linear Models; Lipopeptides - blood; Liquid chromatography; Medical sciences; Micafungin; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Wavelengths
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2011.03.008

A direct-injection HPLC-based method has been developed for determining amounts of micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column. The method is easy to perform and requires only 10 μL of a filtered plasma sample. The chromatographic separations were carried out with a gradient mode. The fluorescence detection wavelengths of excitation and emission were set at 273 nm and 464 nm, respectively. Retention times for micafungin and IS were 22.4 and 23.7 min, respectively. Micafungin and FR195743 (IS) peaks were completely separated with little tailing, and no interference was observed. The calibration curve of micafungin showed good linearity in the range of 0.5–20.0 μg/mL ( r 2 = 1.00). The intra-day accuracy ranged from −4.5 to 5.3%. The inter-day accuracy ranged from −9.8 to 1.5%. The precisions were less than 10%. This method is useful for the determination of micafungin in human plasma.