Proteomic analysis of differentially expressed proteins in Lactobacillus brevis NCL912 under acid stress

Abstract Protein expression of Lactobacillus brevis NCL912 under acid stress was analysed by two-dimensional gel electrophoresis and MS. Twenty-five proteins were differentially expressed under acid stress. Among them, eight protein spots were identified by matrix-assisted laser desorption/ionizatio...

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Bibliographic Details
Published in:FEMS microbiology letters 2011-05, Vol.318 (2), p.177-182
Main Authors: Huang, Guidong, Li, Chaobo, Cao, Yusheng
Format: Article
Language:English
Subjects:
acid stress
Acids
Acids - metabolism
Analytical, structural and metabolic biochemistry
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
DNA biosynthesis
DNA repair
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Gene Expression Regulation, Bacterial
Glycolysis
Ionization
Ions
Lactobacillus brevis
Lactobacillus brevis - chemistry
Lactobacillus brevis - genetics
Lactobacillus brevis - physiology
Lactobacillus brevis NCL912
Microbiology
Miscellaneous
Molecular Sequence Data
mRNA
Protein biosynthesis
Protein synthesis
Proteins
Proteomics
proteomics analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stress response
Stress, Physiological
Two dimensional analysis
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Summary:Abstract Protein expression of Lactobacillus brevis NCL912 under acid stress was analysed by two-dimensional gel electrophoresis and MS. Twenty-five proteins were differentially expressed under acid stress. Among them, eight protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight MS, of which seven were upregulated and one was downregulated. The function of the downregulated protein was unknown and the putative functions of the upregulated proteins were categorized as stress response, DNA repair, protein synthesis and glycolysis. Quantitative real-time PCR was used to further validate these differentially expressed proteins at the mRNA level and a positive correlation between the content of the proteins and their mRNA levels was found. The results suggest that these proteins are involved in the acid stress response mechanisms of this bacterium.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2011.02257.x