Notch1 and the activated NOTCH1 intracellular domain are expressed in differentiated trophoblast cells

The Notch signalling pathway regulates proliferation, cell death and cell type specification that is critical for organogenesis. Mouse models carrying mutations in the Notch signalling pathway display defects in development of the placenta, suggesting that this pathway is required for placental deve...

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Bibliographic Details
Published in:Cell biology international 2011-05, Vol.35 (5), p.443-447
Main Authors: Sarikaya, Didem Pelin, Jerome-Majewska, Loydie Anne
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques
Cell Differentiation
Cell Nucleolus - metabolism
differentiation
Female
FGF4
Fibroblast Growth Factor 4 - metabolism
Mice
Notch1
placenta
Pregnancy
Receptor, Notch1 - analysis
Receptor, Notch1 - genetics
Receptor, Notch1 - metabolism
Stem Cells - cytology
Stem Cells - metabolism
trophoblast stem cell
Trophoblasts - cytology
Trophoblasts - metabolism
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Summary:The Notch signalling pathway regulates proliferation, cell death and cell type specification that is critical for organogenesis. Mouse models carrying mutations in the Notch signalling pathway display defects in development of the placenta, suggesting that this pathway is required for placental development. In particular, Notch1 mutant embryos exhibit abnormal placental morphogenesis and arrest early in development. However, expression of Notch1 gene has not been detected during placental development. Trophoblast stem cells are derived from the precursor of the placenta and express Notch1. We report that Notch1 is also expressed in differentiated trophoblast cells. Under standard differentiation conditions, Notch1 expression ceases by day 6. Furthermore, the activated NOTCH1 intracellular domain is enriched at the nucleolus of trophoblast stem cells and differentiated trophoblast cells. Our results suggest that NOTCH1 is active in both trophoblast stem cells and differentiated trophoblast cells.
ISSN:1065-6995
1095-8355
DOI:10.1042/CBI20100394