Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma

A strategy for the determination of the presence of thiol-containing amino acids was successfully established by simply assembling copper chloride and xylenol orange (3,3'-bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein trisodium salt; XO) in a 1 : 1 molar ratio in quasi-physiol...

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Bibliographic Details
Published in:Analyst (London) 2011-05, Vol.136 (9), p.1892-1897
Main Authors: HUO, Fang-Jun, YANG, Yu-Tao, JING SU, SUN, Yuan-Qiang, YIN, Cai-Xia, YAN, Xu-Xiu
Format: Article
Language:English
Subjects:
Analytical chemistry
Anions - chemistry
Biosensing Techniques - methods
Chemistry
Color
Colorimetry - methods
Copper - metabolism
Cysteine - blood
Exact sciences and technology
General, instrumentation
Glutathione - blood
Homocysteine - blood
Humans
Hydrogen-Ion Concentration
Indicators and Reagents - chemistry
Linear Models
Molecular Structure
Plasma - chemistry
Spectrometric and optical methods
Spectrophotometry, Ultraviolet - methods
Sulfhydryl Compounds - blood
Water - chemistry
Xylenes - metabolism
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Summary:A strategy for the determination of the presence of thiol-containing amino acids was successfully established by simply assembling copper chloride and xylenol orange (3,3'-bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein trisodium salt; XO) in a 1 : 1 molar ratio in quasi-physiological water solution (pH 6.0). The copper(II)-XO ensemble was highly selective for thiol species such as cysteine, homocysteine, and glutathione without interference from other amino acids and could quantitatively detect thiol in the range from 10 to 200 μM with a linear relationship having an average molar absorbance constant of 6530 L mol(-1) cm(-1) in pure water. The whole recognition process for thiol gave rise to a rapid visual color change from purple-red to yellow which can be observed simultaneously with the naked-eye.
ISSN:0003-2654
1364-5528
DOI:10.1039/c1an15014f