Cell Differentiation and Matrix Organization in Engineered Teeth

• Published in: Journal of dental research 2011-05, Vol.90 (5), p.583-589
• Main Authors: Lechguer, A. Nait, Couble, M.L., Labert, N., Kuchler-Bopp, S., Keller, L., Magloire, H., Bleicher, F., Lesot, H.
• Format: Article
• Language: English
• Subjects: Ameloblasts; Ameloblasts - cytology; Ameloblasts - ultrastructure; Amelogenesis; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Biological and medical sciences; Biotechnology; Cell Differentiation; Cell Polarity; Cells, Cultured; Cementogenesis; Cementum; Collagen; Crystallization; Crystals; Dental enamel; Dental prosthetics; Dentin; Dentinogenesis; Dentistry; Electron diffraction; Electron Probe Microanalysis; Embryonic Stem Cells - cytology; Embryonic Stem Cells - transplantation; Enamel; Enamel Organ - cytology; Endoplasmic reticulum; Extracellular Matrix - ultrastructure; Fibroblasts; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Histogenesis; Histology; Industrial applications and implications. Economical aspects; Laboratory animals; Ligaments; Medical sciences; Mice; Mice, Inbred ICR; Microscopy, Electron, Transmission; Mineralization; Miscellaneous; Morphogenesis; Neovascularization, Physiologic; Odontoblasts; Odontoblasts - cytology; Odontoblasts - ultrastructure; Periodontal ligament; Periodontal Ligament - growth & development; Periodontium; Stem Cell Transplantation; Teeth; Tissue Engineering; Tooth Crown - growth & development; Tooth Root - growth & development; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transmission electron microscopy; Vascularization
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034510391796

Embryonic dental cells were used to check a series of criteria to be achieved for tooth engineering. Implantation of cultured cell-cell re-associations led to crown morphogenesis, epithelial histogenesis, organ vascularization, and root and periodontium development. The present work aimed to investigate the organization of predentin/dentin, enamel, and cementum which formed and mineralized after implantation. These implants were processed for histology, transmission electron microscopy, x-ray microanalysis, and electron diffraction. After two weeks of implantation, the re-associations showed gradients of differentiating odontoblasts. There were ciliated, polarized, and extended cell processes in predentin/dentin. Ameloblasts became functional. Enamel crystals showed a typical oriented arrangement in the inner and outer enamel. In the developing root, odontoblasts differentiated, cementogenesis occurred, and periodontal ligament fibroblasts interacted with the root surface and newly formed bone. The implantation of cultured dental cell re-associations allows for reproduction of complete functional differentiation at the cell, matrix, and mineral levels.