Improved Preservation and Microcirculation with POLYSOL After Partial Liver Transplantation in Rats

• Published in: The Journal of surgical research 2011-05, Vol.167 (2), p.e375-e383
• Main Authors: Yagi, Shintaro, M.D., Ph.D, Doorschodt, Benedict Marie, M.D., Ph.D, Afify, Mamdouh, V.V.M., Ph.D, Klinge, Uwe, M.D., Ph.D, Kobayashi, Eiji, M.D., Ph.D, Uemoto, Shinji, M.D., Ph.D, Tolba, Rene H., M.D., Ph.D
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - metabolism; Animals; Bilirubin - metabolism; Cold Temperature; Glucose - therapeutic use; ischemia-reperfusion injury; L-Lactate Dehydrogenase - metabolism; Liver - blood supply; Liver - drug effects; Liver - physiology; liver regeneration; Liver Transplantation - physiology; living-donor liver transplantation; Male; Malondialdehyde - metabolism; Mannitol - therapeutic use; Microcirculation - drug effects; Microcirculation - physiology; Models, Animal; organ preservation; Organ Preservation - methods; Organ Preservation Solutions - pharmacology; Organ Preservation Solutions - therapeutic use; POLYSOL; Potassium Chloride - therapeutic use; Procaine - therapeutic use; Rats; Rats, Inbred Lew; Regeneration - drug effects; Regeneration - physiology; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Surgery
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2010.12.040

Background Due to the severe shortage of deceased donors, demand for living-donor liver transplantation (LDLT) has increased worldwide. Here, we compared POLYSOL, a recently developed low-viscosity preservation solution, and histidine-tryptophan-ketoglutarate (HTK) for cold storage of partial liver graft in this study. Methods Partial liver transplantations with 30% of the native liver were performed in Lewis rats. The graft livers were flushed with either HTK or POLYSOL ( n = 25, respectively) and stored in the respective solution for 3 h at 5°C. Graft function was evaluated regarding ischemia-reperfusion injury and regeneration at 1, 3, 24, and 168 h after reperfusion. Results POLYSOL preservation resulted in improvement of portal venous flow (HTK versus POLYSOL; mean ± SEM: 16.8 ± 2.2 versus 21.6 ± 2.1 mL/min; P = 0.005), microcirculation (383 ± 63 versus 532 ± 64 Flux; P = 0.045), ALT (310.2 ± 56.1 versus 181.8 ± 17.0 IU/L; P = 0.0262), LDH (4052.4 ± 764.4 versus 2494.1 ± 410.0 IU/L; P = 0.0215), total bilirubin (21.6 ± 14.2 versus 4.0 ± 0.6 IU/L; P = 0.0236), malondialdehyde (100.0 ± 4.3 versus 69.2 ± 4.0 nmol/mL; P = 0.0015), as well histologic findings at 24 h. Liver regeneration was improved in POLYSOL with regards to liver weight (4.0 ± 0.2 versus 4.3 ± 0.3 g; P = 0.038) and Ki-67 labeling index (9.67 ± 2.17 versus 1.10 ± 0.14%; P