Influence of 2% chlorhexidine gel on calcium hydroxide ionic dissociation and its ability of reducing endotoxin

Objective The aim of this study was to evaluate the influence of 2% chlorhexidine gel (CHX) on pH, calcium release, and Ca(OH)2 capability of reducing endotoxin. Study design Calcium release was verified by atomic-absorbance spectrophotometry, and pH was measured with a pHmeter. For endotoxin quanti...

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Published in:Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2011-05, Vol.111 (5), p.653-658
Main Authors: Signoretti, Fernanda Graziela Correa, MSc, de Almeida Gomes, Brenda Paula Figueiredo, PhD, Montagner, Francisco, PhD, Barrichello Tosello, Fernanda, BDS, Jacinto, Rogério Castilho, PhD
Format: Article
Language:English
Subjects:
Analysis of Variance
Anti-Infective Agents, Local - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiseptics
Biological and medical sciences
Calcium - analysis
Calcium hydroxide
Calcium Hydroxide - chemistry
Chlorhexidine
Chlorhexidine - pharmacology
Dentistry
Endotoxins
Endotoxins - analysis
Humans
Hydrogen-Ion Concentration
Hydroxides - analysis
Limulus Test
Medical sciences
Otorhinolaryngology. Stomatology
pH effects
Pharmacology. Drug treatments
Root Canal Irrigants - chemistry
root canals
Spectrophotometry
Statistical analysis
Statistics, Nonparametric
Surgery
Teeth
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Summary:Objective The aim of this study was to evaluate the influence of 2% chlorhexidine gel (CHX) on pH, calcium release, and Ca(OH)2 capability of reducing endotoxin. Study design Calcium release was verified by atomic-absorbance spectrophotometry, and pH was measured with a pHmeter. For endotoxin quantification, extracted human teeth previously contaminated with standard endotoxin were filled with: group I, Ca(OH)2 + saline solution; group II, Ca(OH)2 + CHX; and group III, CHX for 14 days. The remaining endotoxin was quantified by using chromogenic quantitative test. Statistical analysis was performed with analysis of variance and Tukey post hoc test ( P < .05). Results Group II released more calcium than group I ( P < .05) after 15 days. Groups I and II showed alkaline pH in all periods, and group I showed higher pH values than group II ( P < .05) after 30 days. Groups II and III showed significantly more decreased endotoxin than group I. Conclusions CHX did not interfere with the chemical properties of Ca(OH)2 , in fact even improving its properties of reducing the endotoxin content in root canals in vitro.
ISSN:1079-2104
1528-395X
DOI:10.1016/j.tripleo.2010.11.016