Human mammaglobin transcript amplification for differential diagnosis in a breast cancer metastatic to dura mater

In breast cancer (BC), metastases to the central nervous system usually arise in women with advanced disease. Diagnosis of leptomeningeal (LM) metastasis is based on neurological symptoms, imaging studies and cytological detection of malignant cells in the cerebrospinal fluid (CSF). However, often t...

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Bibliographic Details
Published in:Anticancer research 2011-03, Vol.31 (3), p.1061-1064
Main Authors: Dono, Mariella, Ferro, Paola, Franceschini, Maria Cristiana, Dessanti, Paolo, Bacigalupo, Bartolomeo, Cibei, Eugenio, Capellini, Cesare, Amoroso, Domenico, Camerini, Andrea, Fedeli, Franco, Roncella, Silvio
Format: Article
Language:English
Subjects:
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Diagnosis, Differential
Dura Mater - pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Magnetic Resonance Imaging
Mammaglobin A
Meningeal Neoplasms - diagnosis
Meningeal Neoplasms - genetics
Meningeal Neoplasms - secondary
Middle Aged
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Uteroglobin - genetics
Uteroglobin - metabolism
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Summary:In breast cancer (BC), metastases to the central nervous system usually arise in women with advanced disease. Diagnosis of leptomeningeal (LM) metastasis is based on neurological symptoms, imaging studies and cytological detection of malignant cells in the cerebrospinal fluid (CSF). However, often these approaches are not sensitive enough to recognize leptomeninges involvement and subsequently to make a diagnosis of LM carcinomatosis. This study investigated the employment of reverse transcriptase-polymerase chain reaction (RT-PCR) for the human mammaglobin (hMAM) gene in a case of BC with cerebral metastases in which the involvement of the leptomeninges was in doubt. Amplification of hMAM mRNA was performed from CSF cells by RT-PCR. No amplification of hMAM was obtained from the CSF cells. RT-PCR for human mammaglobin mRNA of the CSF in BC patients with brain metastases may aid clinical determination of LM involvement and consequently the choice of the most effective therapy regimens for affected patients.
ISSN:1791-7530