Effects of simvastatin on cytokines secretion from mononuclear cells from critically ill patients with acute kidney injury

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2011-05, Vol.54 (2), p.144-148
• Main Authors: Ferrari, Gabriela Laender, Redublo Quinto, Beata Marie, Batista de Souto Queiroz, Kelly Cristina, Iizuka, Ilson Jorge, Martins Monte, Julio Cesar, Dalboni, Maria Aparecida, Durão, Marcelino Souza, Neto, Miguel Cendoroglo, Pavão dos Santos, Oscar Fernando, Batista, Marcelo Costa
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - metabolism; AKI; Case-Control Studies; centrifugation; Critical Illness; Cytokines; Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology; Inflammation; interleukin-10; Interleukin-10 - secretion; kidneys; Monocytes - drug effects; Monocytes - secretion; mononuclear leukocytes; patients; secretion; Sepsis; Simvastatin; Simvastatin - pharmacology; Tumor Necrosis Factor-alpha - secretion
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2011.02.005

To assess the in vitro effects of simvastatin on IL-10 and TNF-α secretion from peripheral blood mononuclear cells (PBMC) of critically ill patients with and without acute kidney injury (AKI). PBMC were collected from 63 patients admitted to the intensive care unit (ICU) and from 20 healthy controls. Patients were divided in 3 subgroups: with AKI, with sepsis and without AKI and with AKI and sepsis. After isolation by ficoll-gradient centrifugation cells were incubated in vitro with LPS 1ng/mL, simvastatin (10−8M) and with LPS plus simvastatin for 24h. TNF-α and IL-10 concentrations on cells surnatant were determined by ELISA. Cells isolated from critically ill patients showed a decreased spontaneous production of TNF-α and IL-10 compared to healthy controls (6.7 (0.2–12) vs 103 (64–257) pg/mL and (20 (13–58) vs 315 (105–510) pg/mL, respectively, p