Genetic diversity and relationship of endangered plant Magnolia officinalis (Magnoliaceae) assessed with ISSR polymorphisms

Twenty-eight populations of the rare medicinal plant Magnolia officinalis (Magnoliaceae) were sampled across its natural range, and inter-simple sequence repeats (ISSRs) markers were used to assess the genetic variation within and among populations. Twelve primer combinations produced a total of 137...

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Bibliographic Details
Published in:Biochemical systematics and ecology 2011-04, Vol.39 (2), p.71-78
Main Authors: Yu, Hua-Hui, Yang, Zhi-Ling, Sun, Bo, Liu, Ruo-nan
Format: Article
Language:English
Subjects:
anthropogenic activities
gene flow
genes
genetic background
Genetic diversity
genetic drift
Genetic relationship
genetic variation
ISSR
loci
Magnolia
Magnolia officinalis
Magnoliaceae
medicinal plants
microsatellite repeats
species diversity
variance
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Summary:Twenty-eight populations of the rare medicinal plant Magnolia officinalis (Magnoliaceae) were sampled across its natural range, and inter-simple sequence repeats (ISSRs) markers were used to assess the genetic variation within and among populations. Twelve primer combinations produced a total of 137 unambiguous bands of which 114 (83.2%) were polymorphic. M. officinalis exhibited a relatively low genetic diversity at population level (the percentage of polymorphic loci PPB = 49.8%, Nei’s genetic diversity H = 0.194, Shannon’s information index I = 0.286). However, the genetic diversity at species level was relatively high ( PPB = 83.2%; H = 0.342; I = 0.496). The coefficient of gene differentiation ( G ST, 42.8%) and the results of analysis of molecular variance (AMVOA) indicated that genetic differentiation occurred mainly within populations. The estimated gene flow ( Nm) from G ST was 0.669. It indicated that the fragmentation and isolation of populations might result from specific evolutionary history and anthropogenic activity. Genetic drift played a more important role than gene flow in the current population genetic structure of M.  officinalis. Conservation strategies for this rare species are proposed based on the genetic data. ► This study reports on genetic diversity in the endangered species, M. officinalis. ► ISSR markers used to identify genetic variation and population differentiation. ► M. officinalis had low population-level genetic diversity. ► 666 individuals from 28 populations in China over a large geographical range was analyzed. ► This study is a very thorough investigation of the population genetics of M. officinalis.
ISSN:0305-1978
1873-2925
DOI:10.1016/j.bse.2010.12.003