Effect of Artemisinin Combined with Glucocorticoid on the Expressions of Glucocorticoid Receptor α mRNA,Glucocorticoid Receptor β mRNA and P300/CBP Protein in Lupus Nephritis Mice

• Published in: Chinese journal of integrative medicine 2011-04, Vol.17 (4), p.277-282
• Main Author: 吴喜利 张王刚 石兴民 安鹏 孙万森 乔成林 王竹
• Format: Article
• Language: English
• Subjects: Animals; Artemisinins - administration & dosage; Artemisinins - pharmacology; Base Sequence; Disease Models, Animal; DNA Primers; Electrophoresis, Agar Gel; Female; Lupus Nephritis - genetics; Lupus Nephritis - metabolism; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; mRNA; Original Article; p300-CBP Transcription Factors - metabolism; Prednisone - administration & dosage; Prednisone - pharmacology; Receptors, Glucocorticoid - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; 受体结合; 外周血单个核细胞; 糖皮质激素受体; 蛋白表达; 雌性小鼠; 青蒿素
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-011-0693-3

Objective：To investigate the therapeutic effects and mechanisms of using artemisinin（Art） combined with glucocorticoid（GC） to treat lupus nephritis（LN） mice.Methods：Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table：control group,model group,prednisone group administrated with 6.45 mg/（kg·d） prednisone suspension,and Art＋prednisone group administrated with 150 mg/（kg·d） Art suspension and 3.225 mg/（kg·d） prednisone suspension.A mice model of LN was established by injection with living lymph cell suspension.The changes of urine protein/24h,the expressions of GC receptorα（GRα） mRNA,GC receptorβ（GRβ） mRNA in peripheral blood mononuclear cells（PBMCs）,and transcriptional coactivator P300/CBP protein in renal tissue were measured.Results： Compared with the model group,the treatment groups had significant decrease in urine protein/24 h,and renal pathological lesion（P0.01）.In the same groups,the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRαmRNA were significantly increased,and GRβmRNA expression was significantly decreased（P0.01）.And the Art＋prednisone group has a better therapeutic effect than the prednisone group （P0.01）.Conclusions：Art has therapeutic sensitization effects on GC in the LN mice.The underlying mechanism could be correlated with the effect of Art on the increase of the expressions of GRαmRNA and transcriptional coactivator P300/CBP protein in renal tissue and on the decrease of the expression of GRβmRNA in PBMCs.