Multiplex PCR for detection of acquired carbapenemase genes

Abstract A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes. Primers were designed to amplify the following 11 genes: blaIMP , blaVIM , blaNDM , blaSPM , blaAIM , blaDIM , blaGIM , blaSIM blaKPC , blaBIC , and blaOXA-4...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2011-05, Vol.70 (1), p.119-123
Main Authors: Poirel, Laurent, Walsh, Timothy R, Cuvillier, Vincent, Nordmann, Patrice
Format: Article
Language:English
Subjects:
Bacteria - enzymology
Bacteria - genetics
Bacterial Proteins - genetics
beta-Lactamases - genetics
Biological and medical sciences
Carbapenem
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Medical sciences
Metallo-β-lactamase
Microbiology
Multiplex
Polymerase Chain Reaction - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes. Primers were designed to amplify the following 11 genes: blaIMP , blaVIM , blaNDM , blaSPM , blaAIM , blaDIM , blaGIM , blaSIM blaKPC , blaBIC , and blaOXA-48 . Three different multiplex reaction mixtures were defined and evaluated for the detection of all these 11 genes. Using optimized conditions, each reaction mixture allowed to identify the respective genes, with PCR giving distinct amplicon sizes corresponding to the different genes for each mixture. We reported here a rapid and reliable technique for screening all clinically relevant carbapenemase genes.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2010.12.002