HPLC–FLD determination of serum aromatic amino acids: Application in chronic kidney disease patients

The aromatic amino acids (AAA) are very important amino acids in the body and have been suggested to be involved in many diseases. We describe the development and full validation of a high performance liquid chromatography with fluorescence detection (HPLC–FLD) method for simultaneously quantitative...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 2011-05, Vol.412 (11-12), p.1032-1035
Main Authors: Li, Ying, Tang, Ai-Guo, Mu, Sa
Format: Article
Language:English
Subjects:
Adolescent
Adult
Amino Acids, Aromatic - blood
Aromatic amino acids
Blood Chemical Analysis - methods
Case-Control Studies
Chromatography, High Pressure Liquid - methods
Chronic Disease
Chronic kidney disease
Female
High performance liquid chromatography
Humans
Kidney Diseases - blood
Limit of Detection
Linear Models
Male
Middle Aged
Phenylalanine
Spectrometry, Fluorescence - methods
Time Factors
Tryptophan
Tyrosine
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aromatic amino acids (AAA) are very important amino acids in the body and have been suggested to be involved in many diseases. We describe the development and full validation of a high performance liquid chromatography with fluorescence detection (HPLC–FLD) method for simultaneously quantitative determination of serum AAA. Furthermore, we aimed to explore the clinical significances of AAA for chronic kidney disease (CKD). Serum samples were deproteinized by perchloric acid. Separation was carried out on a Megres C18 column (4.6mm×250mm i.d., 5μm). The mobile phase consisted of 10% acetonitrile (v/v) in water and was run at a flow rate of 1.0ml/min. The eluted AAA was monitored by measuring the fluorescence intensity using the programmed wavelength detection setting. AAA determination in a serum sample was achieved in
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2011.02.015