The electrochemiluminescence of ruthenium complex/tripropylamine systems at DNA-modified gold electrodes

The electrochemiluminescence (ECL) behavior of ruthenium complex/tripropylamine (TPA) systems at DNA-modified gold electrode was studied to understand the possible mechanism and to develop new detection platforms. DNA strand, especially double-stranded DNA (ds-DNA), can preconcentrate TPA and acts a...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2703-2706
Main Authors: Liu, Dong-Yuan, Xin, You-Ying, He, Xi-Wen, Yin, Xue-Bo
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensor
Biosensors
Biotechnology
Computer-Aided Design
DNA
DNA - chemistry
DNA-modified electrode
Electrochemiluminescence
Electrodes
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
gold
Gold - chemistry
luminescence
Luminescent Measurements - instrumentation
lysozyme
Mechanism
Methods. Procedures. Technologies
oligonucleotides
Propylamines - chemistry
protons
ruthenium
Ruthenium - analysis
Ruthenium - chemistry
Various methods and equipments
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Summary:The electrochemiluminescence (ECL) behavior of ruthenium complex/tripropylamine (TPA) systems at DNA-modified gold electrode was studied to understand the possible mechanism and to develop new detection platforms. DNA strand, especially double-stranded DNA (ds-DNA), can preconcentrate TPA and acts as the acceptor of the protons released from TPAH +, therefore the improved ECL emission and the low potential ECL were observed. The intercalation of Ru(phen) 3 2+ into ds-DNA was confirmed to be a sensitive and label-free DNA-related detection platform. The above results were validated by the analysis of lysozyme using anti-lysozyme aptamer-modified electrode. This work opens a new field by the use of DNA-modified electrode to develop novel sensing platforms, such as low potential ECL biosensors and Ru(phen) 3 2+ intercalation-based ECL biosensors.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.08.074