Using silicon nanowire devices to detect adenosine triphosphate liberated from electrically stimulated HeLa cells

In this study, we used a biosensor chip featuring Abl tyrosine kinase-modified silicon nanowire field-effect transistors (SiNW-FETs) to detect adenosine triphosphate (ATP) liberated from HeLa cells that had been electrically stimulated. Cells that are cultured in high-ionic-strength media or buffer...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2323-2328
Main Authors: Chen, C.C., Chen, Y.-Z., Huang, Y.-J., Sheu, J.-T.
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Adenosine Triphosphate - biosynthesis
ATP
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Buffer solutions
Chips
Conductometry - instrumentation
Electric Stimulation - methods
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
HeLa cell
HeLa Cells
Humans
Ionic strength
Microelectrodes
Nanocomposites
Nanomaterials
Nanotechnology - instrumentation
Nanotubes - chemistry
Nanotubes - ultrastructure
Nanowires
Silicon - chemistry
Silicon nanowire field-effect transistor
Transistors, Electronic
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Summary:In this study, we used a biosensor chip featuring Abl tyrosine kinase-modified silicon nanowire field-effect transistors (SiNW-FETs) to detect adenosine triphosphate (ATP) liberated from HeLa cells that had been electrically stimulated. Cells that are cultured in high-ionic-strength media or buffer environments usually undermine the sensitivity and selectively of SiNW-FET-based sensors. Therefore, we first examined the performance of the biosensor chip incorporating the SiNW-FETs in both low- and high-ionic-strength buffer solutions. Next, we stimulated, using a sinusoidal wave (1.0 V, 50 Hz, 10 min), HeLa cells that had been cultured on a cell-culture chip featuring interdigitated electrodes. The extracellular ATP concentration increased by ca. 18.4-fold after electrical stimulation. Finally, we detected the presence of extracellular ATP after removing a small amount of buffer solution from the cell-cultured chip and introducing it into the biosensor chip.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.10.003