A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase

Collagen/calcium phosphate composite materials were prepared using an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. We prepared a collagen/calcium phosphate composite film by adding sequentially calcium chloride, disodium glycerophosphat...

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Published in:Journal of the Ceramic Society of Japan 2008, Vol.116(1349), pp.10-13
Main Authors: TOMOMATSU, Ohki, TACHIBANA, Akira, YAMAUCHI, Kiyoshi, TANABE, Toshizumi
Format: Article
Language:English
Subjects:
Alakaline phosphatase
Collagen
Composite
Hydroxyapatite
Mineralization
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TACHIBANA, Akira
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TANABE, Toshizumi
description Collagen/calcium phosphate composite materials were prepared using an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. We prepared a collagen/calcium phosphate composite film by adding sequentially calcium chloride, disodium glycerophosphate and alkaline phosphatase to collagen solution and subsequent drying of the resultant composite suspension in a template. The calcium phosphate contained in the film was determined to be mainly hydroxyapatite from FT-IR and X-ray diffraction analysis. The film little swelled in water to keep its original morphology, while a collagen film became solubilized. This suggests the binding between collagen and calcium phosphate in complex materials. When the preosteoblast cell line, MC3T3-E1 cells were cultured on the film, the film was found to support the osteoblastic differentiation from the measurement of alkaline phosphatase activity, which is an early differentiation marker.
doi_str_mv 10.2109/jcersj2.116.10
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source J-STAGE (Japan Science & Technology Information Aggregator, Electronic) - Open Access English articles
subjects Alakaline phosphatase
Collagen
Composite
Hydroxyapatite
Mineralization
title A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase
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