DNA hybridization sensor based on pentacene thin film transistor

A DNA hybridization sensor using pentacene thin film transistors (TFTs) is an excellent candidate for disposable sensor applications due to their low-cost fabrication process and fast detection. We fabricated pentacene TFTs on glass substrate for the sensing of DNA hybridization. The ss-DNA (polyA/p...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2264-2269
Main Authors: Kim, Jung-Min, Jha, Sandeep Kumar, Chand, Rohit, Lee, Dong-Hoon, Kim, Yong-Sang
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
Conductometry - instrumentation
Deoxyribonucleic acid
Devices
Disposable sensor
DNA hybridization sensor
Electrical properties
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
In Situ Hybridization - instrumentation
Label-free
Membranes, Artificial
Naphthacenes - chemistry
Pentacene
Pentacene thin film transistors
Semiconductor devices
Sensors
Thin film transistors
Transistors, Electronic
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Summary:A DNA hybridization sensor using pentacene thin film transistors (TFTs) is an excellent candidate for disposable sensor applications due to their low-cost fabrication process and fast detection. We fabricated pentacene TFTs on glass substrate for the sensing of DNA hybridization. The ss-DNA (polyA/polyT) or ds-DNA (polyA/polyT hybrid) were immobilized directly on the surface of the pentacene, producing a dramatic change in the electrical properties of the devices. The electrical characteristics of devices were studied as a function of DNA immobilization, single-stranded vs. double-stranded DNA, DNA length and concentration. The TFT device was further tested for detection of λ-phage genomic DNA using probe hybridization. Based on these results, we propose that a “label-free” detection technique for DNA hybridization is possible through direct measurement of electrical properties of DNA-immobilized pentacene TFTs.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.09.047