Hemodiafiltration With Online Regeneration of Ultrafiltrate: Effect on Heme-Oxygenase-1 and Inducible Subunit of Nitric Oxide Synthase and Implication for Oxidative Stress and Inflammation

Hemodiafiltration with regeneration of ultrafiltrate (HFR) has a positive impact on inflammation and oxidative stress (OxSt), risk factors for cardiovascular disease (CVD), the most common cause of excess morbidity and mortality for end‐stage renal disease (ESRD) patients. However, studies have been...

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Bibliographic Details
Published in:Artificial organs 2011-02, Vol.35 (2), p.183-187
Main Authors: Calò, Lorenzo A., Naso, Agostino, Davis, Paul A., Pagnin, Elisa, Corradini, Robert, Tommasi, Adalberto, Sereni, Luisa, Ragazzi, Eugenio
Format: Article
Language:English
Subjects:
Adult
Aged
Artificial organs
Cardiovascular risk
Dialysis
Female
Heme Oxygenase-1 - metabolism
Heme-oxygenase-1
Hemodiafiltration
Hemodiafiltration - methods
Humans
Inflammation - prevention & control
Interleukin-1beta - blood
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - metabolism
Male
Middle Aged
Nitric oxide
Nitric Oxide Synthase Type II - immunology
On-line systems
Online
Oxidative Stress
Regeneration
Stresses
Young Adult
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Summary:Hemodiafiltration with regeneration of ultrafiltrate (HFR) has a positive impact on inflammation and oxidative stress (OxSt), risk factors for cardiovascular disease (CVD), the most common cause of excess morbidity and mortality for end‐stage renal disease (ESRD) patients. However, studies have been of limited duration. This study extends our previous study of HFR effects by evaluating the effect on mononuclear cell protein expression of heme‐oxygenase‐1 (HO‐1), induced by OxSt, and inducible subunit of nitric oxide synthase (iNOS), and plasma level of interleukin‐1β (Il‐1β) and oxidized low‐density lipoproteins (OxLDL), marker of OxSt, for a 12‐month period. Fourteen ESRD patients stable on hemodialysis over a period of at least 2 years and on conventional bicarbonate dialysis were switched to be treated with HFR. Blood samples were collected at baseline, after 3, 6, 9 and 12 months. HO‐1 and iNOS protein expression were evaluated by Western blot, OxLDL by enzyme‐linked immunosorbent assay (ELISA), and Il‐1β by enzyme amplified sensitivity immumoassay assay. HFR significantly increased HO‐1 at the 9 and 12 months (ANOVA = P 
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2010.01045.x