Potential of protoporphyrin IX and metal derivatives for single molecule fluorescence studies

Metalloporphyrins are cofactors of a variety of proteins, and are often used as spectroscopic probes of the active site. Many high resolution techniques, such as single molecule spectroscopy, are based on fluorescence contrast and require the replacement of the native metalloporphyrin by a fluoresce...

Full description

Saved in:
Bibliographic Details
Published in:Journal of luminescence 2011-03, Vol.131 (3), p.477-481
Main Authors: Hu, Yi, Geissinger, Peter, Woehl, Jörg C.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Atomic and molecular physics
Biological and medical sciences
Cross sections
Derivatives
Exact sciences and technology
Fluorescence
Fluorescence and phosphorescence spectra
Fluorescence and phosphorescence
radiationless transitions, quenching (intersystem crossing, internal conversion)
Fluorescence quantum yield
Fundamental and applied biological sciences. Psychology
High resolution
Luminescence
Metalloporphyrins
Miscellaneous
Molecular absorption
Molecular absorption cross section
Molecular properties and interactions with photons
Physics
Proteins
Single molecule spectroscopy
Spectroscopy
Zinc
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Metalloporphyrins are cofactors of a variety of proteins, and are often used as spectroscopic probes of the active site. Many high resolution techniques, such as single molecule spectroscopy, are based on fluorescence contrast and require the replacement of the native metalloporphyrin by a fluorescent analog. We have investigated the potential of several fluorescent analogs of heme, namely free-base protoporphyrin IX and its metal derivatives containing Zn, Sn, and Mg, for single molecule fluorescence studies by determining their room-temperature molecular absorption cross sections and fluorescence quantum yields. According to these data, free-base protoporphyrin IX and its Zn derivative, which have the highest fluorescence quantum yields, are the most suitable heme analogs for single molecule fluorescence studies. ► Protoporphyrin IX and fluorescent metal derivatives for single molecule detection. ► Measurement of room temperature absorption cross sections for Q bands. ► Measurement of room temperature fluorescence quantum yields for Q bands. ► PPIX and Zn derivative have highest quantum yields for lowest-energy transition.
ISSN:0022-2313
1872-7883
DOI:10.1016/j.jlumin.2010.12.012