A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants

In this study, bacteria hosted in root nodules of single plants of legume Arachis hypogaea L. (peanut) cv Tegua Runner growing at field were isolated. The collection of nodule isolates included both fast and slow growing strains. Their genetic diversity was assessed in order to identify the more fre...

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Bibliographic Details
Published in:Current microbiology 2011-06, Vol.62 (6), p.1752-1759
Main Authors: Angelini, Jorge, Ibáñez, Fernando, Taurian, Tania, Tonelli, María Laura, Valetti, Lucio, Fabra, Adriana
Format: Article
Language:English
Subjects:
Arachis - microbiology
Bacteria
Biodiversity
Biomedical and Life Sciences
Biotechnology
Bradyrhizobium - classification
Bradyrhizobium - genetics
Bradyrhizobium - isolation & purification
Bradyrhizobium - physiology
Flowers & plants
Genetic diversity
Heterogeneity
Legumes
Life Sciences
Microbiology
Molecular Sequence Data
Nitrogen Fixation
Peanuts
Phylogeny
Root Nodules, Plant - microbiology
Soil Microbiology
Symbiosis
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Summary:In this study, bacteria hosted in root nodules of single plants of legume Arachis hypogaea L. (peanut) cv Tegua Runner growing at field were isolated. The collection of nodule isolates included both fast and slow growing strains. Their genetic diversity was assessed in order to identify the more frequently rhizobial strain associated to nodules from single plants. Molecular fingerprinting of 213 nodular isolates indicated heterogeneity, absence of a dominant genotype and, therefore, of a unique strains highly competitive. Efficient nitrogen-fixing isolates were identified as Bradyrhizobium sp. by phylogenetic analysis of the sequences of their 16S rRNA genes. The genetic diversity of 68 peanut nodulating isolates from all the collected plants was also analyzed. Considering their ERIC-PCR profiles, they were grouped in eighteen different OTUs for 60% similarity cut-off. Results obtained in this study indicate that the genetic diversity of rhizobia occupying nodules from single plant is very high, without the presence of a dominant strain. Therefore, the identification of useful peanut rhizobia for agricultural purposes requires strongly the selection, among the diverse population, of a very competitive genotype in combination with a high-symbiotic performance.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-011-9924-2