Physiologically Stressed Cells of Fluorescent Pseudomonas EKi as Better Option for Bioformulation Development for Management of Charcoal Rot Caused by Macrophomina phaseolina in Field Conditions

Bioformulation that supports the inoculant under storage condition and on application to field is of prime importance for agroindustry. Pseudomonas strain EKi having biocontrol activity against Macrophomina phaseolina was used in the study. EKi cells were pretreated by carbon starvation, osmotic str...

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Bibliographic Details
Published in:Current microbiology 2011-06, Vol.62 (6), p.1789-1793
Main Authors: Khare, Ekta, Arora, Naveen K
Format: Article
Language:English
Subjects:
Agricultural industry
Anatomy & physiology
Antibiosis
Ascomycota - physiology
bacteria
Biological control
Biomedical and Life Sciences
Biotechnology
Carbon
Cells
Cicer - growth & development
Cicer - microbiology
Cicer arietinum
Coal
environmental factors
Fluorescence
Freeze drying
Life Sciences
Macrophomina phaseolina
Microbiology
osmotic stress
Pest Control, Biological - methods
Plant Diseases - microbiology
Plant growth
Proteins
Pseudomonas
Pseudomonas - physiology
shelf life
Sodium chloride
starvation
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Summary:Bioformulation that supports the inoculant under storage condition and on application to field is of prime importance for agroindustry. Pseudomonas strain EKi having biocontrol activity against Macrophomina phaseolina was used in the study. EKi cells were pretreated by carbon starvation, osmotic stress (NaCl), and freeze drying conditions, and talc-based bioformulation was developed. Combined pretreatment with carbon starvation and osmotic stress was given to Pseudomonas cells. Bioformulation of untreated, freeze dried (FD), carbon starved, osmotic stressed, and combined pre-treated cells showed 50.36, 44.76, 45.95, 34.82, and 27.27% reduction in CFU counts after 6 months of storage. The osmotic stressed cells showed one over-expressed protein (11.5 kDa) in common with carbon starved cells responsible for its better shelf life. The plant growth promotory activity of bioformulations was determined taking Cicer arietinum as a test crop in M. phaseolina infested field. Carbon starved + osmotic stressed cells showed maximum enhancement of dry weight (272.56%) followed by osmotic stressed (230.74%), untreated (155.70%), FD (88.93%), and carbon starved (59.34%) cells over uninoculated control. Carbon starved + osmotic stressed, osmotic stressed, untreated, FD, and carbon starved cells showed 156.60, 100, 75, 40, and 16.67% reduction of charcoal rot disease over uninoculated control. The results clearly showed that combined pretreatment by carbon starvation and osmotic stress provides the bacteria potential of rapid adaptation to different environment conditions.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-011-9929-x