Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population

The 14-bp polymorphism in exon 8 of the HLA-G gene is associated with HLA-G mRNA stability and the patterns of alternative isoform splicing and may influence the functionality of the HLA-G molecule. HLA-G expression was related to allograft acceptance and fewer episodes of acute rejection during hea...

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Bibliographic Details
Published in:Molecular biology reports 2011-06, Vol.38 (5), p.3593-3597
Main Authors: Aghdaie, Mahdokht H., Azarpira, Negar, Kazemi, Kurosh, Geramizadeh, Bita, Darai, Masumeh, Malekhoseini, Seid Ali
Format: Article
Language:English
Subjects:
Adult
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Exons
Female
Genetic markers
Genotype
Genotype & phenotype
Graft Rejection - genetics
Graft Rejection - immunology
Histocompatibility Antigens Class I - genetics
Histology
HLA Antigens - genetics
HLA-G Antigens
Humans
Iran
Kidney Transplantation - immunology
Kidneys
Life Sciences
Male
Molecular biology
Morphology
Polymorphism
Polymorphism, Genetic
RNA Stability - genetics
Transplantation, Homologous - immunology
Transplants & implants
Treatment Outcome
Young Adult
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Summary:The 14-bp polymorphism in exon 8 of the HLA-G gene is associated with HLA-G mRNA stability and the patterns of alternative isoform splicing and may influence the functionality of the HLA-G molecule. HLA-G expression was related to allograft acceptance and fewer episodes of acute rejection during heart, kidney and liver–kidney transplantation. In order to determine a possible correlation between the 14-bp insertion/deletion polymorphism and kidney allograft outcome in our population, genomic DNA was isolated from 144 patients who had received isolated kidney allografts. The recipients was divided into two groups, grafts presenting features of rejection group and a non-rejection group, and compared them with a control group of 100 healthy subjects. There was no significant difference in allelic frequencies of 14-bp insertion/deletion polymorphism between normal controls and kidney transplant patients. No significant difference was found between the RG and the NRG regarding the 14-bp genotypes and alleles. Therefore, additional studies with more sample size from other populations with analysis of other HLA-G polymorphisms are necessary to define this polymorphism as a valuable clinical marker.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-010-0470-y